基于MAGIC群体的烟草抗黑胫病全基因组关联分析  

Genome-Wide Association Study for Black Shank Disease Resistance in Tobacco Based on the MAGIC Population

作　　者：潘怡 刘洪泰 吉白璐 王红 黄立钰 程立锐[2] PAN Yi;LIU Hongtai;JI Bailu;WANG Hong;HUANG Liyu;CHENG Lirui(School of Agriculture,Yunnan University,Yunnan,Kunming 650504,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266101,China;Yunnan Tobacco Company Baoshan Company Tengchong Branch,Yunnan,Baoshan 679100,China)

机构地区：[1]云南大学农学院,云南昆明650504 [2]中国农业科学院烟草研究所,山东青岛266101 [3]云南省保山市烟草公司腾冲分公司,云南保山679100

出　　处：《热带农业科学》2025年第2期72-79,共8页Chinese Journal of Tropical Agriculture

基　　金：云南省教育厅科学研究基金项目“烟草黑胫病抗病基因的克隆与功能验证”(No.2023Y0231);中国烟草总公司科技重大专项项目“烟草重要香气性状全基因组选择模型构建及育种利用”[No.110202101040(JY-17)]。

摘　　要：由烟草疫霉引起的黑胫病是烟草主要病害之一,严重影响烟叶的产量和品质。为了进一步明确烟草黑胫病抗性遗传基础,发掘烟草黑胫病抗性位点,采用8亲本组配的MAGIC(Multiparent Advanced Generation Inter-Cross)群体为试验材料,在苗期和大田期调查亲本及后代群体的黑胫病抗性;利用430K烟草SNP(SingleNucleotidePoly-morphism)芯片鉴定基因型,并对烟草黑胫病不同生育期抗性进行全基因组关联分析(Genome-Wide Association Study,GWAS)。结果显示,在苗期和大田期重复定位到2个与黑胫病病情指数及发病率相关的黑胫病抗性主效QTL(Quantitative Trait Locus),分别位于7号和14号染色体,最显著SNP物理位置分别在7号染色体175557310 bp和14号染色体7074846bp;其中,7号染色体定位结果与前人研究结果一致,14号染色体定位结果为烟草黑胫病抗性新位点。本研究为深入挖掘烟草抗黑胫病主效基因和开展抗性分子标记辅助改良提供参考。Black shank,one of the paramount diseases affecting the yield and quality of tobacco,is caused by Phytophthora nicotianae.To further clarify the genetic basis of black shank disease resistance and discover new resistance loci for this disease,a MAGIC(Multiparent Advanced Generation Inter-Cross)population with eight parents was used as the material,and the resistance of the parental and offspring populations'resistance to black shank disease was investigated at the seedling and field stages.We identified the genotypes of the MAGIC population via a 430 K tobacco SNP(Single-Nucleotide Polymorphism)chip and conducted a genome-wide association study(GWAS)for tobacco black shank resistance.The results revealed that two major QTLs(Quantitative Trait Locus)related to the disease index and incidence rate of black shank resistance,which are located on chromosomes 7 and 14,were repeatedly identified at the seedling stage and field stage.The most significant SNPs were physically located on chromosome 7 at 175557310 bp and chromosome 14 at 7074846 bp,respectively.The localization of chromosome 7 is in line with the results of previous studies,and the localization of chromosome 14 is a new locus for tobacco black shank disease resistance.The results of this study provide reference for further exploration of major genes conferring resistance to tobacco black shank disease and the implementation of resistance molecular marker-assisted improvement.

关 键 词：MAGIC群体 烟草 黑胫病 GWAS 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]