巴西橡胶树HbPP2基因家族鉴定与表达分析  

Identification and Expression Analysis of HbPP2 Gene Family in Hevea brasiliensis

作　　者：杨那 林远 胡斌 郝园园 韩晓菲[1] 刘林娅 龙翔宇[3] YANG Na;LIN Yuan;HU Bin;HAO Yuanyuan;HAN Xiaofei;LIU Linya;LONG Xiangyu(School of Life and Health,Dalian University,Dalian,Liaoning 116622,China;School of Biological Science and Technology,Liupanshui Normal University,Liupanshui,Guizhou 553004,China;Institute of Rubber Research,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China)

机构地区：[1]大连大学生命健康学院,辽宁大连116622 [2]六盘水师范学院生物科学与技术学院,贵州六盘水553004 [3]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2025年第4期777-787,共11页Chinese Journal of Tropical Crops

基　　金：六盘水师范学院科研培育项目(No.LPSSY2023KJZDPY07);六盘水师范学院科研项目(No.LPSSYLPY202226);海南省自然科学基金创新团队项目(No.322CXTD526)。

摘　　要：橡胶树胶乳代谢过程受到多种机制调控,其中泛素化修饰调控最为显著。韧皮部蛋白PP2属于F-box蛋白家族,在胶乳中特异性高表达,可能参与了胶乳再生调控。本研究对巴西橡胶树基因组中鉴定到的17个F-box成员HbPP2基因进行生物信息学和表达模式分析,结果表明:橡胶树PP2基因编码的蛋白大多数为不稳定亲水性蛋白,分子量介于9.402~61.206 kDa之间;系统进化分析表明巴西橡胶树中的17个HbPP2基因可以分为3个亚组(Ⅰ亚组,Ⅱ亚组和Ⅲ亚组),其中HbPP2成员在Ⅰ亚组中完全缺失;顺式作用元件分析发现,PP2基因启动子区域包含生长发育调控、激素响应和光响应等多种顺势作用元件;亚细胞定位预测发现,PP2蛋白主要定位在叶绿体或细胞质中;表达分析显示,橡胶树HbPP2家族中除部分成员具有组织表达特异性外,多数成员在各个组织中均有表达,在胶乳中,HbPP2-B1、HbPP2-B1.1、HbPP2-B10.1、HbPP2-A13.2、HbPP2-B13和HbPP2-A15.1的表达丰度较高,但割胶处理只显著上调HbPP2-A13.2和HbPP2-B13的表达。本研究初步揭示巴西橡胶树HbPP2家族成员的理化特征和表达特征,为进一步研究该基因家族在橡胶树胶乳合成代谢中的功能奠定基础。Rubber tree latex metabolic processes are regulated by a variety of mechanisms,among which ubiquitination modification is the most significant.The phloem protein PP2 belongs to the F-box protein family and is specifically highly expressed in latex,which may be involved in the regulation of latex regeneration.In this study,bioinformatics and expression pattern analysis were conducted on 17 F-box members of the HbPP2 gene identified in the genome of Hevea brasiliensis.The results revealed that the majority of proteins encoded by the rubber tree PP2 genes were unsta-ble hydrophilic proteins with molecular weights ranging from 9.402 to 61.206 kDa.Phylogenetic analysis showed that the 17 HbPP2 genes in the H.brasiliensis could be divided into three subgroups(Subgroup Ⅰ,Subgroup Ⅱ,Subgroup Ⅲ),in which HbPP2 members were completely absent in subgroup Ⅰ.Analysis of cis-acting elements revealed that the rubber tree PP2 gene family contained diverse functional elements involved in growth and development regulation,hormone response,and light response.Subcellular localization analysis indicated that PP2 proteins were primarily localized in chloroplasts or cytoplasm.Tissue expression analysis showed that,except for some members with tissue-specific expression,most members of the rubber tree HbPP2 family were expressed in various tissues.In latex,the expression levels of six genes,HbPP2-B1,HbPP2-B1.1,HbPP2-B10.1,HbPP2-A13.2,HbPP2-B13 and HbPP2-A15.1,were relatively high,but tapping treatment significantly upregulated the expression of HbPP2-A13.2 and HbPP2-B13.This study would provide preliminary insights into the physicochemical and expression characteristics of the HbPP2 family members in the H.brasiliensis,laying a foundation for further research on the functions of this genes family in rubber trees latex synthesis and metabolism.

关 键 词：巴西橡胶树 基因表达分析 韧皮部蛋白2 PP2基因家族 胶乳生物合成 

分 类 号：S794.1[农业科学—林木遗传育种]