芒果果实MiERF20基因的克隆、定位及表达分析  

Cloning,Localization and Expression Analysis of MiERF20 Gene in Mango Fruit

作　　者：郭盼 王鑫 张莎莎[1,2] 马德新 龚艳 邵远志 李雯 GUO Pan;WANG Xin;ZHANG Shasha;MA Dexin;GONG Yan;SHAO Yuanzhi;LI Wen(School of Tropical Agriculture and Forestry,Hainan University,Danzhou,Hainan 571737,China;Sanya Institute of Southern Propagation,Hainan University,Sanya,Hainan 572025,China;Hainan Haiken Cold Chain Development Co.,Ltd.,Sanya,Hainan 572013,China)

机构地区：[1]海南大学热带农林学院,海南儋州571737 [2]海南大学三亚南繁研究院,海南三亚572025 [3]海南海垦冷链发展有限公司,海南三亚572013

出　　处：《热带作物学报》2025年第4期788-797,共10页Chinese Journal of Tropical Crops

基　　金：海南农垦联合创新科研项目(No.HKKJ202432);海南大学芒果产业体系建设项目。

摘　　要：芒果(Mangifera indica L.)是典型的呼吸跃变型水果,其成熟衰老与乙烯密切相关。ERF转录因子(ethyleneresponsive factor)在植物生长发育、果实成熟和乙烯信号转导中发挥重要作用。本研究以贵妃芒果品种为试验材料,克隆MiERF20基因,并对其生物学功能进行分析。结果表明:MiERF20基因全长492 bp,编码163个氨基酸;MiERF20蛋白为不稳定的亲水蛋白,无信号肽和跨膜区,含有25个磷酸化位点及1个AP2保守结构域;其二级结构以40.49%的无规则卷曲为主,还含有33.13%的α-螺旋、16.56%的延伸链和9.82%的β-转角,三级结构符合AP2结构域特征;MiERF20与漆树科开心果的ERF20蛋白同源性达83.89%。亚细胞定位显示MiERF20位于细胞核,且具有转录激活活性。原核表达实验表明该蛋白在原核系统中能被诱导表达。实时荧光定量分析结果显示,MiERF20基因受乙烯诱导表达,表明其在乙烯调控果实成熟中发挥重要作用。本研究为进一步解析ERF转录因子在果实采后成熟过程中的功能和调控机制提供基础。Mango(Mangifera indica L.),as a typical climacteric fruit,undergoes ripening and senescence processes closely related to ethylene.Ethylene-responsive factors(ERF)play crucial roles in plant growth,fruit ripening and ethylene signaling transduction.Mango variety Guifei was used,MiERF20,492 bp long and encoding 163 amino acids,was cloned and analyzed.Bioinformatics analysis revealed that MiERF20 was an unstable,hydrophilic protein without signal peptides or transmembrane regions,containing 25 phosphorylation sites and one AP2 conserved domain.The secondary structure was dominated by 40.49% irregular coils,33.13% α-helices,16.56%extensions and 9.82% β-rotations.Its secondary structure comprised irregular coils,α-helices,extended chains,andβ-turns,consistent with AP2 domain features.MiERF20 showed 83.89%homology with ERF20 of Lacertidae pistachio.Subcellular localization indicated that MiERF20 was located in the nucleus and possessed transcriptional activation activity.Prokaryotic expression assays demonstrated that the protein could be inducibly expressed in a prokaryotic system.Real-time fluorescence quantification showed that MiERF20 was ethylene-inducible,suggesting its role in ethylene-regulated fruit ripening.This study would provide a foundation for further analysis of the functions and regulatory mechanisms of ERF transcription factors during postharvest fruit ripening.

关 键 词：芒果 ERF转录因子 乙烯信号 基因表达 表达分析 

分 类 号：S667.7[农业科学—果树学]