西番莲HSF基因家族的全基因组鉴定及成花期对高温胁迫的响应  

Genome-wide Identification of HSF Gene Family and Their Response to Hot Ambient Temperature During Flower Development in Passion Fruit(Passiflora edulis)

作　　者：吴艳艳 刘洁云 周俊妞 田青兰 黄伟华 朱陈利 牟海飞 WU Yanyan;LIU Jieyun;ZHOU Junniu;TIAN Qinglan;HUANG Weihua;ZHU Chenli;MOU Haifei(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西壮族自治区农业科学院生物技术研究所,广西南宁530007 [2]广西壮族自治区农业科学院,广西南宁530007

出　　处：《热带作物学报》2025年第4期807-819,共13页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.32260740,No.32060660);广西自然科学基金项目(No.2023GXNSFAA026301)。

摘　　要：热激转录因子(heat shock transcription factor,HSF)是重要的调节因子,在植物响应高温胁迫中发挥重要作用。高温胁迫对西番莲成花造成了严重的负面影响,因此研究HSF调控西番莲耐热性的意义重大。本研究利用生物信息学技术在西番莲基因组上鉴定了26个热激转录因子基因,分别命名为PeHSF1~PeHSF26。西番莲HSF基因编码蛋白长度为100~1589个氨基酸,分子量为11544.9~177157.8 g/mol,理论等电点为4.25~10.80。西番莲26个HSF基因分布在第1、4、5、6、7、8和9染色体上,分为10个亚群。西番莲全基因组复制事件分析结果表明,ZX01G0033270、ZX01G0052790和ZX01G0052980等5个HSF基因位于重复片段内。西番莲HSF基因有1~12个内含子和2~13个外显子,都包含HSF-DNA结合结构域。利用RNA-seq分析黄果原生种与金陵紫果HSF基因对高温环境的响应,发现ZX01G0002530、ZX01G0052790和ZX01G0121040等基因在花蕾中表达量差异显著,并通过RT-qPCR验证候选HSF基因表达水平。综合分析,推测西番莲响应高温胁迫的最理想的候选HSF基因为ZX01G0121040。这些结果有助于了解HSF在西番莲成花期响应高温胁迫过程中的功能,为西番莲耐高温的遗传改良提供重要基因资源和理论依据。Heat shock transcription factor(HSF)is an important regulatory factor that plays a crucial role in plant response to hot ambient temperature(HAT).High temperature stress has a serious negative impact on the flowering of passion fruit,therefore,studying the regulation of heat tolerance by HSF in passion fruit is of great significance.In this study,we identified 26 HSF genes on the genome of passion fruit using bioinformatics technology,named PeHSF1-PeHSF26.The genes could encode proteins of 100-1589 amino acids,with molecular weight 11544.9-177157.8 g/mol,and a theoretical isoelectric point of 4.25-10.80.The 26 genes were distributed on chromosomes 1,4,5,6,7,8 and 9,and were divided into 10 subpopulations.The analysis of the whole genome replication event showed that ZX01G0033270,ZX01G0052790 and ZX01G0052980 were located within the genome duplications.The genes contained 1-12 introns and 2-13 exons,all contained HSF-DNA binding domains.RNA-seq analysis of Huangguo original species and Jinlingziguo HSF genes to HAT revealed that there were significant differences in the expression levels of genes,including ZX01G0002530,ZX01G0052790 and ZX01G0121040 in flower buds.The expression levels of candidate HSF genes were validated by RT-qPCR.Based on comprehensive analysis,it is supposed that the most ideal candidate HSF gene for passion flower response to high temperature stress was ZX01G0121040.The results would contribute to understand the function of HSF in response to high temperature stress during the flowering of passion fruit,and provide important genetic resources and theoretical basis for genetic improvement of passion fruit.

关 键 词：西番莲 高温胁迫 热激转录因子 同源基因 成花 转录组测序 候选基因 

分 类 号：Q945.78[生物学—植物学] S667.9[农业科学—果树学]