刈草种林对滨海湿地土壤氨氧化微生物丰度的影响  

Effects of Replacing Spartina alterniflora with Kandelia obovata on Abundance of Soil Ammonia-oxidizing Microorganism in Coastal Wetland

作　　者：叶瑞超 叶桂萍 杨平 冯蒙蒙[1,2] 韩风毅 成于恒 贺纪正 林永新 YE Ruichao;YE Guiping;YANG Ping;FENG Mengmeng;HAN Fengyi;CHENG Yuheng;HE Jizheng;LINYongxin(Cultivation Base of State Key Laboratory for Subtropical Mountain Ecology,Fuzhou 350007,China;School of Geographical Sciences,Fujian Normal University,Fuzhou 350007,China;School of Geography and Oceanography,Minjiang University,Fuzhou 350108,China;Southeast Ecological Fragile Area Monitoring and Restoration Engineering Innovation Center,Ministry of Natural Resources,Fuzhou 350001,China)

机构地区：[1]湿润亚热带山地生态国家重点实验室培育基地,福州350007 [2]福建师范大学地理科学学院,福州350007 [3]闽江学院地理与海洋学院,福州350108 [4]自然资源部东南生态脆弱区监测修复工程技术创新中心,福州350001

出　　处：《土壤》2025年第1期124-131,共8页Soils

基　　金：国家自然科学基金项目(41930756);福建省自然资源科技创新项目(KY-090000-04-2022-012);闽江学院引进人才预研项目(MJY20012)资助。

摘　　要：依托福建省闽江河口鳝鱼滩湿地建立的短叶茳芏本地植物、互花米草入侵和互花米草刈割种植红树林(刈草种林)样地,利用实时定量PCR方法,研究互花米草入侵和刈草种林对土壤氨氧化微生物丰度的影响。结果表明,植被类型和季节变化均显著影响土壤理化性质,互花米草入侵提高土壤硝态氮含量,刈草种林则降低硝态氮含量。冬季土壤pH、有机碳、全氮和铵态氮含量均显著高于夏季,盐度则显著低于夏季。植被类型显著影响氨氧化古菌(AOA)和氨氧化细菌(AOB)丰度,主要表现为刈草种林显著降低AOA丰度,但显著增加冬季AOB丰度。虽然植被类型对完全氨氧化菌的影响相对较小,但互花米草入侵显著降低冬季完全氨氧化菌clade A丰度。季节变化仅显著影响AOB丰度,主要表现为3种植被类型冬季的AOB丰度均高于夏季。综上,刈草种林生态恢复措施显著降低AOA丰度,提高冬季AOB丰度,且降低土壤硝态氮含量,可能对土壤的硝化过程产生重要影响。This study aimed to examine the impact of Spartina alterniflora invasion and the replacement of Spartina alterniflora with Kandelia obovata(K.obovata restoration)on the abundance of soil ammonia-oxidizing microorganisms through real-time quantitative PCR analysis.Soil samples were collected from a native Cyperus malaccensis site,an area invaded by S.alterniflora,and a site undergoing K.obovata restoration in the Mingjiang River estuary,Fujian Province.The results revealed that significant impacts of both plant species and seasonal variations on soil physicochemical properties,wherein S.alterniflora invasion increased nitrate content,whereas K.obovata restoration decreased it.Soil pH,organic carbon,total nitrogen and ammonium nitrogen contents exhibited significant increases during winter compared to summer,while salinity demonstrated the opposite trend.Plant species had a significant impact on the abundance of ammonia-oxidizing archaea(AOA)and bacteria(AOB),with K.obovata restoration decreasing AOA abundance while increasing AOB abundance in winter.Although the influence of plant species on comammox Nitrospira was comparatively weaker,S.alterniflora invasion substantially reduced the abundance of comammox Nitrospira clade A.Seasonal variations significantly influenced the abundance of AOB,which exhibited higher levels during winter compared to summer across all three plant species.In conclusion,K.obovata restoration led to a significant reduction in AOA abundance and an increase in AOB abundance during winter,along with a decrease in soil nitrate content,potentially influencing soil nitrification process.

关 键 词：河口湿地 植被类型 季节 氨氧化微生物 完全氨氧化菌 

分 类 号：S154.36[农业科学—土壤学]