葛根黄酮的低共熔溶剂提取及其降血糖活性研究  

Research on the Extraction of Puerarin Flavonoids Using Low Eutectic Solvents and Their Hypoglycemic Activity

作　　者：蔡红 李婷 王一男 张国强 CAI Hong;LI Ting;WANG Yinan;ZHANG Guoqiang(Department of Pharmacy,Xuzhou Central Hospital,Xuzhou 221009,China;Department of Clinical Pharmacy,Xuzhou Traditional Chinese Medicine Hospital,Xuzhou 221000,China;School of Pharmacy,Xuzhou Medical University,Xuzhou 221004,China)

机构地区：[1]徐州市中心医院药剂科,江苏徐州221009 [2]徐州市中医院临床药学部,江苏徐州221000 [3]徐州医科大学药学院,江苏徐州221004

出　　处：《特产研究》2025年第2期128-134,共7页Special Wild Economic Animal and Plant Research

基　　金：2023年度徐州市医药卫生项目(KC23187)。

摘　　要：采用低共熔溶剂(Deep eutectic solvents,DES)提取葛根中的黄酮类成分,并研究葛根黄酮对2型糖尿病db/db小鼠的降血糖作用。采用水浴加热法制备DES,超声辅助提取葛根中的黄酮类成分;以葛根素、大豆素、染料木素的含量为指标,通过单因素考察筛选最佳提取工艺;通过测定空腹血糖值(Fasting blood glucose,FBG)和口服葡萄糖耐量试验(Oral glucose tolerance test,OGTT),考察葛根黄酮调节血糖的作用。以氯化胆碱苹果酸制备的DES对葛根中黄酮类成分的提取效率最高,优化后葛根黄酮的提取工艺为:氯化胆碱苹果酸(1:3)、DES的含水量15%、料液比为30 mg/mL、提取时间25 min;葛根黄酮可以有效改善db/db小鼠的高血糖水平,并增强了葡萄糖耐受性。氯化胆碱苹果酸制备的DES可用于葛根中黄酮类成分的高效、快速提取,其DES提取液可用于改善db/db小鼠血糖水平。To extract flavonoids from Pueraria lobata using deep eutectic solvent(Deep eutectic solvents,DES)and to investigate the hy-poglycemic effect of Pueraria flavonoids on db/db mice with type 2 diabetes.DES was prepared using a water bath heating method,and fla-vonoids were extracted from Pueraria lobata with ultrasonic-assisted extraction.The optimal extraction process was screened through single-factor tests based on the content of puerarin,daidzein,and genistein.The hypoglycemic effects of Pueraria flavonoids were assessed by measuring fasting blood glucose(Fasting blood glucose,FBG)levels and conducting an oral glucose tolerance test(Oral glucose toler-ance test,OGTT).The DES composed of choline chloride and malic acid demonstrated the highest extraction efficiency for flavonoids from Pueraria lobata.The optimized extraction conditions were as follows:choline chloride-malic acid(1:3),15%water content in DES,a ma-terial-to-solvent ratio of 30 mg/mL,and an extraction time of 25 minutes.Pueraria flavonoids effectively improved hyperglycemia in db/db mice and enhanced glucose tolerance.The DES prepared from choline chloride and malic acid is suitable for the efficient and green extrac-tion of flavonoids from Pueraria lobata,and the DES extract can improve blood glucose levels in db/db mice with type 2 diabetes.

关 键 词：葛根 黄酮 低共熔溶剂 高效液相色谱法 葛根素 大豆素 染料木素 超声 

分 类 号：R284.1[医药卫生—中药学]