环介导等温扩增技术在动物细小病毒检测中的应用研究进展  

Research Progress of Loop-mediated Isothermal Amplification Technology in Animal Parvovirus Detection

作　　者：赵志刚 郭晓芹 赵传芳[2] 吕文雪[2] 于小亚 吕爽[2] ZHAO Zhigang;GUO Xiaoqin;ZHAO Chuanfang;LV Wenxue;YU Xiaoya;LV Shuang(Suning Agriculture Bureau,Cangzhou 062350,China;Institution of Special Animal and Plant Sciences of CAAS,Changchun 130112,China;Science and Technology Innovation Platform Management Center of Jilin Province,Chan-gchun 130012,China)

机构地区：[1]河北省肃宁县农业农村局,河北沧州062350 [2]中国农业科学院特产研究所,吉林长春130112 [3]吉林省科技创新平台管理中心,吉林长春130012

出　　处：《特产研究》2025年第2期194-199,共6页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技厅创新平台(基地)和人才专项项目(20230508009RC);中国农业科学院特产研究所基本科研业务费项目(1610342023001)。

摘　　要：细小病毒能够引起多种动物发生烈性的传染病,具有高发病率和高死亡率。环介导等温扩增(Loop-mediated isothermal ampli-fication,LAMP)技术是一种新兴的体外核酸扩增技术,因其成本低廉、灵敏度和特异性良好,在病毒、细菌和寄生虫的病原检测和疾病监测等方面广受青睐。本文介绍了LAMP技术及其在动物细小病毒检测中的应用和研究进展,可为该技术在细小病毒检测中的充分运用提供可靠参考。Parvovirus is one of the powerful infectious diseases that can cause a variety of animal diseases,with high morbidity and mortality.Loop-mediated isothermal amplification(LAMP)technology is an emerging in vitro nucleic acid amplification technology.Because of its low cost,excellent sensitivity and specificity,this technology is widely favored in the detection and monitoring of viruses,bacteria,and para-sites.This study introduces LAMP technology and its progress in animal parvovirus detection,laying a theoretical foundation for the full use of parvovirus detection.

关 键 词：环介导等温扩增技术 细小病毒 检测 

分 类 号：S854.43[农业科学—临床兽医学]