亚热带不同林分类型对土壤团聚体中固氮基因丰度的影响  

Effects of forest types on nitrogen-fixing gene abundance in soil aggregates from a subtropical forest

作　　者：韩风毅 邓米林 宛颂 胥超[2,4] 叶桂萍 贺纪正 林永新 HAN Fengyi;DENG Milin;WAN Song;XU Chao;YE Guiping;HE Jizheng;LIN Yongxin(Cultivation Base of State Key Laboratory for Subtropical Mountain Ecology,Fuzhou 350007,China;School of Geographical Sciences,Fujian Normal University,Fuzhou 350007,China;College of Geography and Oceanography,Minjiang University,Fuzhou 350108,China;Fujian Sanming Forest Ecosystem and Global Change National Observation and Research Station,Sanming 365000,Fujian,China)

机构地区：[1]湿润亚热带山地生态国家重点实验室培育基地,福州350007 [2]福建师范大学地理科学学院,福州350007 [3]闽江学院地理与海洋学院,福州350108 [4]福建三明森林生态系统与全球变化国家野外科学观测研究站,福建三明365000

出　　处：《生态学杂志》2025年第3期869-874,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(42077041和41930756)资助。

摘　　要：随着我国社会发展对林木资源需求的增加,大面积的天然林已转变为次生林或人工林。不同林分类型对生态系统结构和功能产生深刻影响。为了研究森林转换后不同林分类型对固氮基因nifH丰度的影响,我们选取米槠天然林皆伐后重新造林形成的杉木和马尾松两种人工林和米槠天然林择伐后形成的米槠次生林进行研究。结果表明:人工林土壤速效磷(AP)含量和含水量高于次生林,其中,马尾松人工林土壤AP含量显著高于次生林和杉木人工林;马尾松人工林土壤nifH基因丰度为4.26×10^(9)copies·g^(-1),显著高于米槠次生林的2.16×10^(9)copies·g^(-1)和杉木人工林的3.07×10^(9)copies·g^(-1);相关性分析表明,土壤nifH基因丰度与AP含量和含水量呈显著正相关;此外,团聚体粒径显著影响nifH基因丰度,三种林分土壤nifH基因丰度均在粉黏颗粒中最低,小团聚体中最高;马尾松人工林不同粒径团聚体的nifH基因丰度整体上高于米槠次生林和杉木人工林。综上,林分类型和团聚体粒径均显著影响亚热带森林土壤nifH基因丰度,AP含量和含水量是关键影响因素。With the increasing demands for wood resources under the social development,large areas of natural forests in China have been transformed into secondary forests or plantations.Different forest types can have profound effects on ecosystem structure and function.We examined the responses of the abundance of nifH genes in soils and aggregates to forest types,including Cunninghamia lanceolata plantation and Pinus massoniana plantation formed by reforestation after clearcutting of natural Castanopsis carlesii forest,as well as secondary C.carlesii forest formed after selective logging.Results showed that soil available phosphorus(AP)and soil moisture contents in both plantations were higher than those in the secondary forest.P.massoniana plantation had significantly higher AP content than the secondary forest and C.lanceolata plantation.The abundance of nifH genes(4.26×10^(9)copies·g^(-1))in P massoniana plantation was significantly higher than that in secondary forest(2.16×10^(9)copies·g^(-1))and C.lanceolata plantation(3.07×10^(9)copies·g^(-1)).The abundance of nifH genes was significantly and positively correlated with AP and moisture contents in bulk soils.Moreover,aggregate sizes had a significant effect on the abundance of the nifH genes,with the lowest abundance in silt-clay particles and the highest in small macroaggregates in all forest types.The abundance of nifH genes in different sizes of aggregates from the P.massoniana plantation was generally higher than that from the secondary forest and C.lanceolata plantation.Overall,both forest types and soil aggregate sizes significantly influenced the abundance of nifH genes in subtropical forests,with AP and moisture contents as the critical driving factors.

关 键 词：菌根 林分类型 NIFH基因 土壤团聚体 

分 类 号：S714.2[农业科学—林学]