Debut of a responsive chemiluminescent probe for butyrylcholinesterase:Application in biological imaging and pesticide residue detection  

作　　者：Shuaige Bai Shuai Huang Ting Luo Bin Feng Yanpeng Fang Feiyi Chu Jie Dong Wenbin Zeng 

机构地区：[1]Xiangya School of Pharmaceutical Sciences,Central South University,Changsha 410013,China [2]Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases,Central South University,Changsha 410013,China [3]The Molecular Imaging Research Center,Central South University,Changsha 410013,China

出　　处：《Chinese Chemical Letters》2025年第3期222-226,共5页中国化学快报(英文版)

基　　金：the financial support from the National Natural Science Foundation of China(Nos.82272067,81974386,and M-0696);Natural Science Foundation of Hunan Province(Nos.2022JJ80052 and 2022JJ40656);the Innovation Fund for Postgraduate Students of Central South University(No.2023ZZTS0609)。

摘　　要：Butyrylcholinesterase(BChE)is a key enzyme in the metabolism of cholinergic compounds.It has been recognized as a key biomarker for many diseases,including liver diseases and Alzheimer’s disease.However,classical methods for detecting BChE activity suffer from low sensitivity,cumbersome pre-treatment,and poor stability.Chemiluminescence is a promising new method for detecting and imaging the activity of BChE.It has several advantages over traditional methods,including low background interference,high sensitivity,and the absence of external illumination.In this study,we developed a novel BChE-activatable chemiluminescent probe(CL-BChE).It exhibited a significant chemiluminescence enhancement at 525nm upon incubation with BChE.It had a low limit of detection(6.25×10^(−3)U/mL)and was highly selective for BChE.CL-BChE was used to image BChE activity in living cells and tumor-bearing animal models.It was also successfully applied to detect pesticide residue,even under the interference of representative phytochromes and real vegetable samples.Given its high sensitivity,selectivity,and versatility,we believe that CL-BChE will be a promising tool for investigating BChE’s activity in biomedical research as well as other BChE-related scenarios.

关 键 词：BUTYRYLCHOLINESTERASE CHEMILUMINESCENCE Tumor imaging Pesticide residue detecting BIOASSAYS 

分 类 号：O657.3[理学—分析化学]