不同除菌方式对猪小肠肝素效价、废水蛋白含量及微生物菌群的影响  

Effect of Different Sterilization Treatments on Heparin Titer,Protein Content in Wastewater and Microflora in Porcine Small Intestine

作　　者：全润 吴晓龙 种正晨 刘容旭 张俊杰 王语聪 谢智鑫 韩建春[1,2] 刘丹怡 QUAN Run;WU Xiaolong;CHONG Zhengchen;LIU Rongxu;ZHANG Junjie;WANG Yucong;XIE Zhixin;HAN Jianchun;LIU Danyi(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Institute of Green Food Science,Harbin 150028,China;Heilongjiang Hanheng Environmental Protection Technology Co.Ltd.,Harbin 150000,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028 [3]黑龙江翰恒环保科技有限公司,黑龙江哈尔滨150000

出　　处：《肉类研究》2025年第3期18-28,共11页Meat Research

基　　金：黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-C010);马铃薯淀粉加工副产物资源化技术研发项目(SKY20210601);家畜小肠高值化加工关键技术开发及产业化示范项目(GA21B015)。

摘　　要：为减少猪小肠肝素提取过程中的微生物污染,采用微酸性电解水(slightly acidic electrolyzed water,SAEW)对猪小肠进行除菌处理,比较直接浸泡、超声浸泡及喷雾3种除菌方式对猪小肠减菌率、肝素效价及肝素废水蛋白含量的影响,以筛选最佳除菌方式,利用16S rRNA高通量测序分析最佳除菌方式处理后猪小肠微生物菌群的组成和变化。结果表明,在最佳除菌条件下,3种除菌方式的除菌效果差异不明显。其中,喷雾除菌对肝素效价和废水蛋白含量影响较小,故选作猪小肠肝素提取前的除菌方式。16S rRNA高通量测序结果表明,有效氯质量浓度达到75 mg/L后,SAEW喷雾除菌能有效杀灭乳球菌属(Lactococcus)、漫游球菌属(Vagococcus)和嗜冷杆菌属(Psychrobacter)等猪小肠优势菌属,减少猪小肠肝素微生物污染。To reduce microbial contamination during heparin extraction from porcine small intestines,this study employed slightly acidic electrolyzed water(SAEW).The sterilization effects of three SAEW treatments:direct immersion,ultrasonicassisted immersion and spraying were evaluated as well as their influences on the bacterial reduction rate,heparin titer and the protein content of wastewater to screen the best sterilization treatment.16S rRNA high-throughput sequencing was used to analyze the composition and changes of microflora in porcine small intestine after the best sterilization treatment.The results showed that under optimal conditions,there was no significant difference in the sterilization effect of the three methods.Of these,spray treatment had the least effect on the heparin titer and the protein content of wastewater,and was thus selected for sterilization before heparin extraction.High-throughput 16S rRNA gene sequencing revealed that spraying SAEW to an available chlorine concentration of 75 mg/L killed the dominant genera in the porcine small intestine,such as Lactococcus,Vagococcus and Psychrobacter,reducing the microbial contamination of porcine small intestinal heparin.

关 键 词：微酸性电解水 肝素 喷雾除菌 微生物菌群 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]