Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing:Approaches and applications  

作　　者：Zhen Wang Xianni Qi Xinru Ren Yuping Lin Fanli Zeng Qinhong Wang 

机构地区：[1]College of Science&Technology,Hebei Agricultural University,Cangzhou,China [2]Key Laboratory of Engineering Biology for Low-carbon Manufacturing,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,China [3]National Center of Technology Innovation for Synthetic Biology,Tianjin,China [4]College of Life Sciences,Hebei Agricultural University,Baoding,China

出　　处：《mLife》2025年第1期1-16,共16页微生物(英文)

基　　金：supported by the National Key Research and Development Program of China(2021YFC2103300);National Natural Science Foundation of China(32161133019 and 32200067);Major Science and Technology Support Program Project in Hebei Province(24292902Z);Key Research and Development Program Projects of Hebei Province(22322905D);Industry University Research Cooperation Project of Shijiazhuang and University of Hebei province(241490062A);supported by Tianjin Industrial Synthetic Biology Innovation Team.

摘　　要：The yeast Saccharomyces cerevisiae is a well-studied unicellular eukaryote with a significant role in the biomanufacturing of natural products,biofuels,and bulk and value-added chemicals,as well as the principal model eukaryotic organism utilized for fundamental research.Robust tools for building and optimizing yeast chassis cells were made possible by the quick development of synthetic biology,especially in engineering evolution.In this review,we focused on methods and tools from synthetic biology that are used to design and engineer S.cerevisiae's evolution.A detailed discussion was held regarding transcriptional regulation,template-dependent and template-free approaches.Furthermore,the applications of evolved S.cerevisiae were comprehensively summarized.These included improving environmental stress tolerance and raising cell metabolic performance in the production of biofuels and bulk and value-added chemicals.Finally,the future considerations were briefly discussed.

关 键 词：biomanufacturing metabolic performance Saccharomyces cerevisiae stress tolerance synthetic evolution 

分 类 号：Q78[生物学—分子生物学]