The endocytic pathway for absorption of exogenous RNAs in Verticillium dahliae  

作　　者：Chuanhui Liu Chen Cui Guanyin Zhou Feng Gao Jianhua Zhao Huishan Guo Yun Jin 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [2]CAS Center for Excellence in Biotic Interactions,Universityof Chinese Academy of Sciences,Beijing,China [3]Zhongmian Seed Technologies Co.,Ltd,Zhengzhou,China

出　　处：《mLife》2025年第1期45-54,共10页微生物(英文)

基　　金：supported by the National Key R&D Programof China(2022YFD1400800);the National Natural ScienceFoundation of China grant(No.32270204);BiologicalBreeding of Early Maturing and Disease Resistant CottonVarieties(Project No.2023ZD04041).

摘　　要：RNAi technologies have been exploited to control viruses,pests,oomycetes,and fungal phytopathogens that cause disasters inhost plants,including many agronomically significant crops.Double-stranded RNA(dsRNA)or small interfering RNA(siRNA)hasbeen applied as a trigger for trans-kingdom RNAi between hosts and fungi.However,it is unclear what process mediates RNAuptake by fungi.In this study,by using live-cell imaging,we determined that exogenously synthesized RNA or small RNA(sRNA)was indiscriminately absorbed into Verticillium dahliae,a notorious pathogenic fungus.Moreover,the application ofendocytic inhibitors or deletion of endocytic-related genes reduced RNA uptake efficiency,showing that RNA absorption byfungal cells occurs mainly through endocytosis.In addition,we found that the endocytosedfluorescence-labeled RNAs werepartly colocalized with endosome marker genes.Overall,our research concluded that exogenous RNA could be assimilated by V.dahliae through the endocytic pathway.Unraveling this cytological mechanism underlying trans-kingdom RNAi holds significantimportance,especially considering the fact that RNAi-based strategies targeting pathogenic fungi are increasingly prevalent in therealm of crop protection.

关 键 词：endocytic pathway pathogenic fungi RNA uptake small RNA 

分 类 号：S432.1[农业科学—植物病理学]