Genome-wide siRNA library screening identifies human host factors that influence the replication of the highly pathogenic H5N1 influenza virus  

作　　者：Guangwen Wang Li Jiang Jinliang Wang Qibing Li Jie Zhang Fandi Kong Ya Yan Yuqin Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Liling Liu Zhigao Bu Hualan Chen Chengjun Li 

机构地区：[1]Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,China

出　　处：《mLife》2025年第1期55-69,共15页微生物(英文)

基　　金：supported by the National Key Research and DevelopmentProgram of China(2021YFD1800203 and 2021YFD1800204);the National Natural Science Foundation of China(NSFC)(32192453,32272979,and 32172847);the China PostdoctoralScience Foundation(2019M660897);the Innovation Program ofChinese Academy of Agricultural Sciences(CAAS-CSLPDCP-202401);the Earmarked Fund for China Agriculture Re-search System(CARS-41-G12)。

摘　　要：The global dissemination of H5 avian influenza viruses represents a significant threat to both human and animal health.In thisstudy,we conducted a genome-wide siRNA library screening against the highly pathogenic H5N1 influenza virus,leading us tothe identification of 457 cellular cofactors(441 proviral factors and 16 antiviral factors)involved in the virus replication cycle.Gene Ontology term enrichment analysis revealed that the candidate gene data sets were enriched in gene categoriesassociated with mRNA splicing via spliceosome in the biological process,integral component of membrane in the cellularcomponent,and protein binding in the molecular function.Reactome pathway analysis showed that the immune system(up to63 genes)was the highest enriched pathway.Subsequent comparisons with four previous siRNA library screenings revealedthat the overlapping rates of the involved pathways were 8.53%-62.61%,which were significantly higher than those of thecommon genes(1.85%-6.24%).Together,our genome-wide siRNA library screening unveiled a panorama of host cellularnetworks engaged in the regulation of highly pathogenic H5N1 influenza virus replication,which may provide potential targetsand strategies for developing novel antiviral countermeasures.

关 键 词：genome-wide siRNA library screening GO analysis H5N1 influenza virus host cellular factor reactome pathwayanalysis 

分 类 号：R373[医药卫生—病原生物学]