桃JMJ组蛋白去甲基化酶家族基因鉴定及表达分析  

Identification and Expression Analysis of Peach JMJ Histone Demethylase Gene Family

作　　者：汪芸芸 周晖 邱可立 潘海发[2] 盛玉[2] 石佩 谢庆梅 陈红莉[2] 张金云[2] 李大辉[1] WANG Yunyun;ZHOU Hui;QIU Keli;PAN Haifa;SHENG Yu;SHI Pei;XIE Qingmei;CHEN Hongli;ZHANG Jinyun;LI Dahui(College of Life Sciences,Anhui Agricultural University,Hefei 230036,China;Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230001,China)

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽省农业科学院园艺研究所,农业农村部园艺作物种质创制与利用重点实验室(省部共建),合肥230001

出　　处：《园艺学报》2025年第3期575-590,共16页Acta Horticulturae Sinica

基　　金：淮北市重大科技专项(HK2021017);安徽省果树产业技术体系项目(AHNYCYTX-10);现代农业产业技术体系建设专项资助(CARS-30-Z-05)。

摘　　要：为深入了解组蛋白甲基化在桃树生长发育和应对非生物胁迫中的功能,对含有JmjC结构域的组蛋白去甲基化酶(JMJ)家族基因进行了全基因组鉴定。在桃树基因组中鉴定到了21个JMJ,根据它们的系统发育关系分为5个亚家族。这些家族成员之间存在较大的差异,基因外显子数在7~33,蛋白中包含12个保守结构域,并且基因启动子上游区域含有不同数量的胁迫响应、激素响应和发育调控元件。选取PpJMJ5作为诱饵,通过酵母双杂交筛库发现了一个潜在互作蛋白PpCIPK1。通过克隆全长进行酵母双杂交试验,证明PpJMJ5与PpCIPK1存在互作关系,可能在一定程度上干扰钙调磷酸酶B类似蛋白(CBL)与其互作蛋白(CIPK)之间的相互作用。PpJMJ5可能在桃树的非生物胁迫响应中发挥重要作用。In order to understand the function of histone methylation in growth and response to abiotic stress in peach trees,genome-wide identification of histone demethylase(JMJ)family genes containing the JmjC domain was performed.Twenty-one JMJs were identified in the peach genome,which were divided into five subfamilies according to their phylogenetic relationships.There were significant differences among the members of these families,with exons ranging from 7 to 33,proteins containing 12 conserved domains,and the upstream regions of gene promoters containing different numbers of stress response,hormone response,and developmental regulatory elements.Using PpJMJ5 as bait,a potential interacting protein PpCIPK1 was found through yeast two-hybrid screening library.It was proved that the interaction between PpJMJ5 and PpCIPK1 existed,which may interfere with the interaction between calhomodin and its interacting protein CBL-CIPK to some extent.PpJMJ5 may play an important role in the abiotic stress response of peach trees.

关 键 词：桃 组蛋白去甲基化酶 非生物胁迫 JMJ 

分 类 号：S662.1[农业科学—果树学]