印度南瓜GAox家族基因鉴定及其在不同蔓长种质中的表达  

Identification and Phylogenetic Analysis of Gibberellin Oxidase Gene Family and Its Expression Pattern in Main Vine Growth in Cucurbita maxima

作　　者：孙廷珍 疏琴 马玮 史玉滋 张蒙 向成钢 薄凯亮 段颖[1] 王长林[1] SUN Tingzhen;SHU Qin;MA Wei;SHI Yuzi;ZHANG Meng;XIANG Chenggang;BO Kailiang;DUAN Ying;WANG Changlin(State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Biological&Agricultural Sciences,Honghe University,Mengzi,Yunnan 661100,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [2]红河学院生物科学与农学学院,云南蒙自661100

出　　处：《园艺学报》2025年第3期603-622,共20页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31972410);北京市设施蔬菜创新团队项目(BAIC01);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)。

摘　　要：赤霉素氧化酶家族GA2ox、GA3ox和GA20ox是植物激素赤霉素合成代谢途径后期关键酶,具有调控植株茎秆伸长等重要作用。以印度南瓜(Cucurbita maxima)参考基因组数据为基础,对其赤霉素氧化酶(GAox)家族基因进行鉴定,对其理化性质、基因结构、染色体定位、系统发育及其近缘物种基因组间的共线性关系等进行了系统分析,并在不同蔓长的印度南瓜种质中研究了其组织特异性表达模式。共鉴定到印度南瓜基因组中13个GA2ox,9个GA3ox和12个GA20ox基因。系统发育研究表明CmaGAox聚类为4个主要分支,上游启动子区分布有多个光响应、逆境胁迫、激素响应以及生长发育调控元件。CmaGAox在印度南瓜中存在9对串联复制基因和10对大片段复制基因,其中只有1对基因(CmaGA3ox5和CmaGA3ox6)受正向选择(Ka/Ks>1)。共线性分析显示印度南瓜CmaGAox与美洲南瓜(Cucurbita pepo)和中国南瓜(Cucurbita moschata)分别存在47和48对共线性基因对。CmaGAox在果实中特异性表达较强,但CmaGA2ox1和CmaGA2ox4在主茎中表达量较高,并且在短蔓印度南瓜Cma0806中的表达量显著高于长蔓印度南瓜Cma0807,表明二者在主蔓生长调控中可能具有重要作用。GA2ox,GA3ox,and GA20ox are pivotal enzymes in the late stage of gibberellin synthesis,playing crucial roles in regulating plant stem elongation.In this study,the GA2ox,GA3ox,and GA20ox gene family were identified utilizing reference genomic data from Cucurbita maxima.Their physicochemical properties,gene structure,chromosome localization,phylogeny,and genomic collinearity with near-origin species were analyzed.Additionally,tissue-specific expression levels of GA2ox genes were investigated in Cucurbita maxima with varying main vine lengths.A total of 13 GA2ox,9 GA3ox,and 12 GA20ox genes were identified in the Cucurbita maxima genome.Phylogenetic analysis based on multiple amino acid sequence alignments showed these gibberellin oxidase gene family forming four major clustered branches.Promoter analysis identified numerous light-responsive,adversity stress-responsive,and hormone-responsive elements.Intragenomic analysis of GAox genes in Cucurbita maxima revealed 9 pairs of tandem duplicated genes and 10 pairs of large replicating genes.Only one gene pair(CmaGA3ox5 and CmaGA3ox6)exhibited positive selection(Ka/Ks>1),while the rest underwent purifying selection.Genome collinearity analysis with near-origin species Cucurbita pepo and Cucurbita moschata unveiled 47 and 48 pairs of collinearity genes,respectively,between Cucurbita maxima,Cucurbita pepo,and Cucurbita moschata.CmaGAox genes were more specifically expressed in fruit,but CmaGA2ox1 and CmaGA2ox4 were more highly expressed in the main stem.The expression patterns of these two genes were significantly higher in the dwarf inbred line Cma0806 than that in the long vine inbred line Cma0807,indicates their potential roles in vine length regulation in Cucurbita maxima.

关 键 词：印度南瓜 赤霉素氧化酶 基因家族 基因进化 主蔓长度 表达分析 

分 类 号：S642.1[农业科学—蔬菜学]