武汉地区油蟠桃病毒种类的小RNA测序鉴定  

Identification and Analysis of Viruses Infecting Flat Nectarine in Wuhan by Small RNA Sequencing

作　　者：刘勇[1] 王富荣[1] 王会良[1] 艾小艳[1] 朱炜 张杨 甘志猛 龚林忠[1] 何华平[1] LIU Yong;WANG Furong;WANG Huiliang;AI Xiaoyan;ZHU Wei;ZHANG Yang;GAN Zhimeng;GONG Lingzhong;HE Huaping(Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees,Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所,果树种质创新与利用湖北省重点实验室,武汉430064

出　　处：《园艺学报》2025年第3期727-736,共10页Acta Horticulturae Sinica

基　　金：现代农业产业技术体系建设专项资助(CARS-30-02A);湖北省种业高质量发展资金项目(HBZY2023B00502)。

摘　　要：为明确侵染湖北武汉地区油蟠桃的病毒种类,采用小RNA测序技术对采集的5份病害样品进行了鉴定分析。结果表明样品中含有桃相关黄症病毒属病毒(peach-associated luteovirus,PaLV)、桃病毒D(peach virus D,PeVD)和桃潜隐花叶类病毒(peach latent mosaic viroid,PLMVd)。采用RT-PCR技术对测序结果进行了验证,获得的PaLV、PeVD和PLMVd基因序列(GenBank登录号:OP244073、OP244074和OP244075)与GenBank中相应的PaLV分离物T01-2(MK361479)、PeVD分离物T02-1(MK361476)和PLMVd分离物HUEI-Ob166/1-1(MW046337)的核苷酸序列一致性为92.15%~98.59%。进一步利用PaLV、PeVD、PLMVd及其他6种桃病毒特异性引物对109份油蟠桃样品进行RT-PCR检测,仅检测到PLMVd、PaLV和PeVD,检出率依次为33.94%、29.36%和5.50%。PLMVd可能为侵染油蟠桃的优势类病毒。病毒和类病毒复合侵染率为22.93%,高于单一的侵染率,表明复合侵染可能是油蟠桃上病毒、类病毒侵染的主要形式。To determine the viruses infecting flat nectarine in Wuhan,Hubei,five shoot samples with the virus-like symptoms were collected and analyzed by small RNA sequencing.The results showed that peach associated luteovirus(PaLV),peach virus D(PeVD),and peach latent mosaic viroid(PLMVd)were detected.RT-PCR with specific primers was conducted to confirm the result.Partial sequences of PaLV,PeVD and PLMVd were obtained by RT-PCR,sequenced and deposited in GenBank.Sequences of PaLV,PeVD and PLMVd(GenBank accession No.OP244073,OP244074 and OP244075)were 92.15%–98.59%identical with the corresponding nucleotide sequences of PaLV isolate T01-2(MK361479),PeVD isolate T02-1(MK361476)and PLMVd isolate HUEI-Ob166/1-1(MW046337)in GenBank.One hundred and nine flat nectarine samples were collected and detected by RT-PCR with primers specific for PaLV,PeVD,PLMVd and six other peach viruses.Only PLMVd,PaLV and PeVD were detected in these samples,with a detection rate of 33.94%,29.36%and 5.50%,respectively.The highest incidence of PLMVd indicated that it was the dominant viroid infecting flat nectarine in Wuhan.The mixed infection of viruses and viroid was 22.93%,higher than that of a single virus or viroid infection,indicating that mixed infection of virus and viroid was common in flat nectarine.

关 键 词：油蟠桃 病毒 类病毒 小RNA测序 RT-PCR 

分 类 号：S662.1[农业科学—果树学]