藤黄檀抗氧化谱效关系研究  

Study on Antioxidant Spectral Relationship of Tenghuangtan(Dalbergia hancei Benth)

作　　者：丘琴 李鹏飞 覃春萍 秦祖杰 莫惠晴 甄丹丹 张淼 刘媛媛 甄汉深 魏江存 QIU Qin;LI Pengfei;QIN Chunping;QIN Zujie;MO Huiqing;ZHEN Dandan;ZHANG Miao;LIU Yuanyuan;ZHEN Hanshen;WEI Jiangcun(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530000,Guagnxi,China;Guangxi International Zhuang Medical Hospital,Nanning 530000,Guangxi,China)

机构地区：[1]广西中医药大学药学院,广西南宁530000 [2]广西国际壮医医院,广西南宁530000

出　　处：《中华中医药学刊》2025年第4期7-13,I0004,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：国家中医药管理局高水平中医药重点学科(壮药学)建设项目(国中医药人教函[2022]226号);广西壮瑶药重点实验室项目(桂科基字[2014]32号);壮瑶药协同创新中心项目(桂教科研[2013]20号);广西高等学校千名中青年骨干教师教育计划项目(桂教教师[2022]60号);广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号);广西科技基地和人才专项(桂科AD21238031);广西重点研发计划项目(桂科AB21196016);广西中医药重点学科壮药学项目(GZXK-Z-20-64);广西一流学科中药学(民族药学)基金项目(桂教科研[2018]12号);广西中医药大学“桂派杏林青年英才”培养项目(2022C032);广西中医药大学“高层次人才培育创新团队”项目(2022A008);广西中医药多学科交叉创新团队项目(GZKJ2309)。

摘　　要：目的采用高效液相色谱法(High performance liquid chromatography,HPLC)建立藤黄檀的指纹图谱,研究其抗氧化活性的谱效关系。方法建立10批不同产地藤黄檀的HPLC指纹图谱,采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical,DPPH)法和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS]法对藤黄檀进行抗氧化活性作用,采用灰色关联度法(Grey relational analysis,GRA)和偏最小二乘回归分析法(Partial least squares regression analysis,PLSR)研究其谱效关系,进而筛选出其抗氧化物质基础。结果建立了10批不同产地藤黄檀的HPLC指纹图谱共有模式,确定了能够表征藤黄檀特征的15个共有峰。10批不同产地批藤黄檀均具有不同程度的抗氧化活性,并呈剂量依赖性。结论成功建立10批不同产地藤黄檀的HPLC指纹图谱与其抗氧化活性的谱效关系,为深入探究藤黄檀抗氧化药效物质基础和质量标准提供了依据。Objective To establish the fingerprint of Tenghuangtan(Dalbergia hancei Benth)by high performance liquid chromatography(HPLC)and to study the spectral-effect relationship of its antioxidant activity.Method HPLC fingerprints of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were established,and the antioxidant activity of Tenghuangtan(Dalbergia hancei Benth)was investigated by the 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)method and 2,2-biazobis(3-ethylbenzothiazole-6-sulfonic acid)diammonium salt(ABTS)method,and its antioxidant activity was studied by the grey relational analysis(GRA)method and partial least squares regression analysis(PLSR)method was used for its spectral-effect relationship,and then its antioxidant substance base was screened out.Results The HPLC fingerprint patterns of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were established,and 15 peaks were identified to characterize Tenghuangtan(Dalbergia hancei Benth).Ten batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were found to possess antioxidant activities in different degrees in a dose-dependent manner.Conclusion The HPLC fingerprints of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different places were successfully established,which provided the basis for in-depth investigation of the antioxidant efficacy of Tenghuangtan(Dalbergia hancei Benth)and its quality standard.

关 键 词：藤黄檀 指纹图谱 抗氧化 谱效关系 

分 类 号：R282.71[医药卫生—中药学]