当归补血汤对再生障碍性贫血小鼠Th17/Treg细胞平衡的影响  

Effect of Danggui Buxue Decoction(当归补血汤)on Balance of Th17/Treg Cells in Mice with Aplastic Anemia

作　　者：陈修保 徐飞[1,2] 尹曦 陈维达[2] 刘秀娟[1,2] 崔兴 陈泽涛[1,2] CHEN Xiubao;XU Fei;YIN Xi;CHEN Weida;LIU Xiujuan;CUI Xing;CHEN Zetao(First Clinical Medical College,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China)

机构地区：[1]山东中医药大学第一临床医学院,山东济南250355 [2]山东中医药大学附属医院,山东济南250014

出　　处：《中华中医药学刊》2025年第4期41-45,I0008,I0009,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82305142);山东省自然科学基金项目(ZR2020MH354,ZR2017LH073)。

摘　　要：目的观察当归补血汤对再生障碍性贫血小鼠骨髓造血功能的影响,探讨辅助性T淋巴细胞17/调节性T淋巴细胞(T helper lymphocytel 17/T regulatory lymphocyte,Th17/Treg)细胞平衡及相关炎性因子在其中的作用。方法利用射线照射联合异源小鼠淋巴细胞输注的方法制备再生障碍性贫血小鼠模型。建模成功后随机分为模型对照组、芪归1∶1组、芪归3∶1组、芪归5∶1组,设正常对照组,各组灌胃相应药物,1次/d,干预28 d。观察小鼠一般情况、体质量、脾脏指数、外周血基本血液学参数变化,苏木精-伊红(hematoxylin-eosin,HE)染色观察骨髓组织病理情况变化;酶联免疫吸附(enzyme linked immunosorbent assay,ELISA)法检测白细胞介素-17A(interleukin-17A,IL-17A)、白细胞介素-6(interleukin-6,IL-6)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)、白细胞介素-10(interleukin-10,IL-10)等炎性因子蛋白表达水平;流式细胞术检测当归补血汤对小鼠骨髓Th17、Treg细胞比例及Th17/Treg细胞平衡的影响。结果当归补血汤治疗组小鼠药物干预后,一般症状均改善,进食量及饮水量增加,体质量、脾脏指数均明显升高,外周血红细胞、白细胞、血小板计数及血红蛋白浓度明显升高(P<0.05),骨髓造血功能改善,造血组织增生度升高(P<0.05);IL-17A、IL-6蛋白表达明显下调,TGF-β1、IL-10蛋白表达明显上调(P<0.05或P<0.01);骨髓Th17细胞比例明显降低,Treg细胞比例明显升高,Th17/Treg细胞比值显著下降(P<0.05或P<0.01),其中芪归5∶1组效果最佳。结论当归补血汤可改善再生障碍性贫血小鼠骨髓造血功能,其作用机制可能与其调控Th17、Treg细胞比例,干预Th17/Treg细胞平衡,调节IL-17A、IL-6、TGF-β1、IL-10等炎性因子水平有关。Objective To observe the effect of Danggui Buxue Decoction(当归补血汤)on bone marrow hematopoietic function in mice with aplastic anemia(AA),and explore the balance of Th17/Treg cells and the role of related inflammatory factors.Method A mouse model of AA was prepared by combining radiation irradiation with allogeneic mouse lymphocyte infusion.After successful modeling,the participants were randomly divided into a model control group,Huangqi(Astragali Radix)-Danggui(Angelicae Sinensis Radix)1∶1 group,Huangqi(Astragali Radix)-Danggui(Angelicae Sinensis Radix)3∶1 group and Huangqi(Astragali Radix)-Danggui(Angelicae Sinensis Radix)5∶1 group.A normal control group was set up,and each group was given the corresponding drugs by gavage once a day for 28 days.The general condition,body weight,spleen index and basic hematological parameters of peripheral blood in mice were observed,and the pathological changes of bone marrow tissue were observed through HE staining.ELSIA method was used to detect the expression levels of inflammatory factor proteins such as interleukin-17A(IL-17A),interleukin-6(IL-6),transforming growth factor-β1(TGF-β1)and interleukin-10(IL-10).Flow cytometry was used to detect the effects of Danggui Buxue Decoction on the proportion of Th17 and Treg cells and the balance of Th17/Treg cells in mouse bone marrow.Result After the intervention of Danggui Buxue Decoction,in the Danggui Buxue Decoction treatment groups,the general symptoms of the mice improved,the food and water intake increased,the body weight and spleen index significantly increased,the peripheral blood red blood cell,white blood cell,platelet count and hemoglobin concentration significantly elevated(P<0.05),the bone marrow hematopoietic function improved,and the hematopoietic tissue proliferation increased(P<0.05).The protein expressions of IL-17A and IL-6 were significantly down-regulated,while the protein expressions of TGF-β1 and IL-10 were significantly up-regulated(P<0.05 or P<0.01).The proportion of Th17 cells in bone m

关 键 词：当归补血汤 TH17/TREG 炎性因子 再生障碍性贫血 经典名方 

分 类 号：R289.5[医药卫生—方剂学]