外周血中CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD200在人类白细胞抗原B27阳性强直性脊柱炎中的表达及临床意义  

Expression and Clinical Significance of CD19^(+)CD24^(high)CD27^(+)Regulatory B Cells,and CD200 in Human Leukocyte Antigen B27 Positive Ankylosing Spondylitis in Peripheral Blood

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作  者:黄晶 李佳慧 张爱荣 娄峻 HUANG Jing;LI Jiahui;ZHANG Airong;LOU Jun(Department of Clinical Laboratory,Zhumadian Central Hospital,Zhumadian 463000,China;Henan Medical School,Kaifeng 475004,China)

机构地区:[1]驻马店市中心医院医学检验科,河南驻马店463000 [2]河南大学医学院,河南开封475004

出  处:《河南医学研究》2025年第6期1048-1052,共5页Henan Medical Research

摘  要:目的探讨外周血中CD200、CD19^(+)CD24^(high)CD27^(+)调节性B细胞在人类白细胞抗原B27阳性强直性脊柱炎(AS)患者中的表达及其临床意义。方法选取2021年1月至2023年6月驻马店市中心医院收治的85例人类白细胞抗原B27阳性强直性脊柱炎患者作为研究对象。根据AS疾病活动度(BASDAI)将其分为活动组(35例)与稳定组(50例);另选取同期在驻马店市中心医院接受健康体检的40例健康人作为对照组。使用流式细胞仪检测研究对象外周血中CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD200水平。比较3组外周血中CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD200水平及基线资料,使用logistic回归性分析CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD200与疾病活动度的关系,绘制受试者工作特征(ROC)曲线,以曲线下面积(AUC)分析CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD200对AS患者疾病活动度的评估价值。结果对照组外周血中CD19^(+)CD24^(high)CD27^(+)调节性B细胞低于稳定组、活动组,稳定组患者CD19^(+)CD24^(high)CD27^(+)调节性B细胞低于活动组(P<0.05);对照组CD3^(+)、CD4^(+)、CD8^(+)、CD19^(+)细胞中CD200水平高于稳定组、活动组,稳定组CD3^(+)、CD4^(+)、CD8^(+)细胞中CD200水平高于活动组(P<0.05);活动组、稳定组CD19^(+)细胞中CD200水平比较,差异无统计学意义(P>0.05);活动组、稳定组患者在性别、年龄等基线资料方面比较,差异无统计学意义(P>0.05);经logistic回归性检验显示,CD3^(+)、CD4^(+)、CD8^(+)细胞中CD200、CD19^(+)CD24^(high)CD27^(+)调节性B细胞是人类白细胞抗原B27阳性AS患者疾病活动度的影响因素(P<0.05)。绘制ROC发现,CD19^(+)CD24^(high)CD27^(+)调节性B细胞、CD3^(+)、CD4^(+)、CD8^(+)细胞中的CD200对AS疾病活动度具有良好的评估价值,AUC分别为0.830、0.786、0.831、0.886,P<0.05;且联合评估具有高价值,AUC为0.974,P<0.05。结论人类白细胞抗原B27阳性AS患者外周血中CD19^(+)CD24^(hObjective To explore the expression and clinical significance of CD200,CD19^(+)CD24^(high)CD27^(+)regulatory B cells in peripheral blood in patients with human leukocyte antigen B27 positive ankylosing spondylitis(AS).Methods A total of 85 patients with human leukocyte antigen B27 positive ankylosing spondylitis admitted to Zhumadian Central Hospital from January 2021 to June 2023 were selected as the study subjects.According to the disease activity of AS(BASDAI),it was divided into an active group(35 cases)and a stable group(50 cases).Another 40 healthy individuals who underwent health examinations in Zhumadian Central Hospital during the same period were selected as the control group.A flow cytometry was used to detect the levels of CD19^(+)CD24^(high)CD27^(+)regulatory B cells,and CD200 in the peripheral blood of the study subjects.The levels of CD19^(+)CD24^(high)CD27^(+)regulatory B cells,CD200,and baseline data in three groups of peripheral blood were compared.Logistic regression was used to analyze the relationship between CD19^(+)CD24^(high)CD27^(+)regulatory B cells,CD200,and disease activity.A receiver operating characteristic(ROC)curve was drawn and analyzed the evaluation value of CD19^(+)CD24^(high)CD27^(+)regulatory B cells and CD200 on disease activity in AS patients using area under curve(AUC).Results The CD19^(+)CD24^(high)CD27^(+)regulatory B cells in the peripheral blood of the control group were lower than those in the stable group and the active group.The CD19^(+)CD24^(high)CD27^(+)regulatory B cells in the stable group were lower than those in the active group(P<0.05).The levels of CD200 in CD3^(+),CD4^(+),CD8^(+),and CD19^(+)cells in the control group were higher than those in the stable and active groups.The levels of CD200 in CD3^(+),CD4^(+),and CD8^(+)cells in the stable group were higher than those in the active group(P<0.05).The comparison of CD200 levels in CD19^(+)cells between the activity group and the stable group showed no statistical significant difference(P>0.05).There was no s

关 键 词:强直性脊柱炎 人类白细胞抗原B27阳性 疾病活动度 CD200 CD19^(+)CD24^(high)CD27^(+)调节性B细胞 

分 类 号:R446.11[医药卫生—诊断学]

 

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