细粒棘球蚴囊液促进肝星状细胞激活和迁移  

Echinococcus granulosus cyst fluid promotes activation and migration of hepatic stellate cells

作　　者：崔杰 伍希雅 林芷伊[1,2] 蔡克丰 吴正展 熊梓彤 黄延鑫 房福众 辛子睿 张宏伟 CUI Jie;WU Xiya;LIN Zhiyi;CAI Kefeng;WU Zhengzhan;XIONG Zitong;HUANG Yanxin;FANG Fuzhong;XIN Zirui;ZHANG Hongwei(The First Affiliated Hospital of Shihezi University,Shihezi 832000,Xinjiang,China;XPCC Clinical Research Center for Infectious Diseases,Shihezi 832000,Xinjiang,China;Key Laboratory of Prevention and Treatment of High Incidence in Central Asia,National Health Commission,Shihezi 832000,Xinjiang,China)

机构地区：[1]石河子大学第一附属医院,新疆石河子832000 [2]兵团感染性疾病临床研究中心,新疆石河子832000 [3]国家卫生健康委中亚高发病防治重点实验室,新疆石河子832000

出　　处：《中国寄生虫学与寄生虫病杂志》2025年第1期69-75,83,共8页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：兵团南疆重点项目(S2021AB3280);国家卫生健康委中亚高发病防治重点实验室开放基金(KF202105);国家(兵团)科技计划(S2022EB5188)。

摘　　要：目的探究人细粒棘球蚴囊液(CF)对肝星状细胞(HSC)激活和迁移的影响及其作用机制。方法收集细粒棘球蚴病患者的肝病灶组织,使用苏木精-伊红(HE)染色和Masson染色观察肝组织的纤维化情况。分别用不含CF和含有10%、20%、40%CF的培养基体外培养HSC 48 h和72 h,蛋白质免疫印迹(Western blotting)检测α平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原α1(Col1a1)、磷脂酰肌醇3激酶(PI3K)、磷酸化PI3K(p-PI3K)、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)等的相对表达水平,实时荧光定量PCR(qPCR)检测α-SMA、Col1a1和Ⅲ型胶原α1(Col3a1)的mRNA相对转录水平,划痕实验和Transwell实验检测CF对HSC迁移的影响。结果HE染色结果显示,细粒棘球蚴病患者肝病灶组织中的细粒棘球蚴外囊呈透明均一的纤维条索样,Masson染色结果显示细粒棘球蚴外囊壁处有大量的胶原纤维沉积。Western blotting结果显示,干预72 h后,对照组、10%CF组、20%CF组和40%CF组的α-SMA蛋白相对表达水平分别为0.359±0.043、0.641±0.088、0.900±0.084、1.111±0.027,10%CF组、20%CF组、40%CF组均高于对照组(t=5.236、10.050、13.980,均P<0.05);各组Col1a1的相对表达水平分别为0.392±0.057、0.546±0.022、0.854±0.034、1.127±0.057,10%CF组、20%CF组、40%CF组均高于对照组(t=4.228、12.630、20.090,均P<0.05)。qPCR结果显示,干预72 h后,对照组、10%CF组、20%CF组和40%CF组α-SMA的mRNA相对转录水平分别为1.000±0.093、1.437±0.106、1.453±0.105、1.697±0.154,10%CF组、20%CF组、40%CF组均高于对照组(t=4.604、4.769、7.339,均P<0.05);各组Col1a1的mRNA相对转录水平分别为0.856±0.042、1.067±0.049、1.283±0.128、1.582±0.046,20%CF组、40%CF组均高于对照组(t=6.932、11.790,均P<0.05);各组Col1a3的mRNA相对转录水平分别为0.611±0.054、0.908±0.041、1.000±0.045、1.239±0.101,10%CF组、20%CF组、40%CF组均高于对照组(t=5.599、7.346、11.850,均P<0.05)。Western blotting结果显示,对照组、10%CFObjective To explore the effects and mechanisms of Echinococcus granulosus cyst fluid(CF)on the activation and migration of hepatic stellate cells(HSC).Methods Liver lesion tissues of cystic echinococcosis(CE)patients were obtained,and the fibrosis of liver tissues was observed using hematoxylin and eosin(HE)staining and Masson staining.HSCs were cultured in vitro for 48 and 72 hours in culture medium without CF and with 10%,20%and 40%CF,respectively.Western blotting was used to detect the relative protein expression levels ofα-mooth muscle actin(α-SMA),collagen type I alpha 1(Col1a1),phosphatidylinositol 3 kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt)and phosphorylated Akt(p-Akt).Real-time quantitative PCR(qPCR)was used to detect the relative mRNA transcription levels ofα-SMA,Col1a1 and Col3a1.Scratch assay and Transwell assays were con-ducted to assess the effects of CF on HSC migration.Results HE staining showed that the outer capsule of E.granulosus in the liver lesion tissues of CE patients was transparent and homogeneous with fibrous strips,and Masson staining showed a large amount of collagen fiber deposition in the outer capsule.Western blotting results showed that after 72 hours of intervention,the relative protein expression levels ofα-SMA in the control group,10%CF group,20%CF group and 40%CF group were 0.359±0.043,0.641±0.088,0.900±0.084 and 1.111±0.027,respectively.The 10%CF group,20%CF group,and 40%CF group had higher levels than the control group(t=5.236,10.050,13.980;all P<0.05).The relative expression levels of Col1a1 in each group were 0.392±0.057,0.546±0.022,0.854±0.034 and 1.127±0.057,respectively.The 10%CF group,20%CF group and 40%CF group had higher levels than the control group(t=4.228,12.630,20.090;all P<0.05).qPCR results showed that after 72 hours of intervention,the relative mRNA transcription levels ofα-SMA in the control group,10%CF group,20%CF group and 40%CF group were 1.000±0.093,1.437±0.106,1.453±0.105 and 1.697±0.154,respectively.The 10%CF group,

关 键 词：细粒棘球蚴 肝星状细胞 囊液 纤维化 

分 类 号：R532.32[医药卫生—内科学]