Effects of ratios of yak to cattle inocula on methane production and fiber digestion in rumen in vitro cultures  

作　　者：Weiwei Wang Wei Guo Jianxin Jiao Emilio M Ungerfeld Xiaoping Jing Xiaodan Huang Allan A Degen Yu Li Sisi Bi Ruijun Long 

机构地区：[1]State Key Laboratory of Grassland Agro-Ecosystems,College of Ecology,Lanzhou University,Lanzhou 730020,China [2]Key Laboratory of Plateau Mountain Animal Genetics,Breeding and Reproduction,Ministry of Education/College of Animal Science,Guizhou University,Guiyang 550025,China 3 [3]Centro Regional de Investigacion Carillanca,Instituto de Investigaciones Agropecuarias INIA,Vilcún,La Araucanía 4880000,Chile [4]School of Public Health,Lanzhou University,Lanzhou 730020,China [5]Desert Animal Adaptations and Husbandry,Wyler Department of Dryland Agriculture,Blaustein Institutes for Desert Research,Ben-Gurion University of the Negev,Beer Sheva 8410500,Israel

出　　处：《Journal of Integrative Agriculture》2025年第4期1270-1284,共15页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(32072757 and U21A20250)。

摘　　要：Yaks are well-adapted to the harsh environment of the Tibetan Plateau,and they emit less enteric methane(CH_(4))and digest poor-quality forage better than cattle.To examine the potential of yak rumen inoculum to mitigate CH_(4)production and improve digestibility in cattle,we incubated substrate with rumen inoculum from yak(YRI)and cattle(CRI)in vitro in five ratios(YRI:CRI):(1)0:100(control),(2)25:75,(3)50:50,(4)75:25 and(5)100:0 for 72 h.The YRI:CRI ratios of 50:50,75:25 and 100:0 produced less total gas and CH_(4)and accumulated less hydrogen(H_(2))than0:100(control)at most time points.From 12 h onwards,there was a linear decrease(P<0.05)in carbon dioxide(CO_(2))production with increasing YRI:CRI ratio.At 72 h,the ratios of 50:50 and 75:25 had higher dry matter(+7.71%and+4.11%,respectively),as well as higher acid detergent fiber digestibility(+15.5%and+7.61%,respectively),when compared to the 0:100 ratio(P<0.05).Increasing the proportion of YRI generally increased total VFA concentrations,and,concomitantly,decreased the proportion of metabolic hydrogen([2H])incorporated into CH_(4),and decreased the recovery of[2H].The lower[2H]recovery indicates unknown[2H]sinks in the culture.Estimated Gibbs free energy changes(ΔG)for reductive acetogenesis were negative,indicating the thermodynamic feasibility of this process.It would be beneficial to identify:1)the alternative[2H]sinks,which could help mitigate CH_(4)emission,and 2)core microbes involved in fiber digestion.This experiment supported lower CH_(4)emission and greater nutrient digestibility of yaks compared to cattle.Multi-omics combined with microbial culture technologies developed in recent years could help to better understand fermentation differences among species.

关 键 词：yak rumen fluid methane DIGESTIBILITY metabolic hydrogen 

分 类 号：S823[农业科学—畜牧学]