Fine mapping and discovery of MIR172e,a candidate gene required for inflorescence development and lower floret abortion in maize ear  

作　　者：Lanjie Zheng Qianlong Zhang Huiying Liu Xiaoqing Wang Xiangge Zhang Zhiwei Hu Shi Li Li Ji Manchun Ji Yong Gu Jiaheng Yang Yong Shi Yubi Huang Xu Zheng 

机构地区：[1]State Key Laboratory of Wheat and Maize Crop Science/Center for Crop Genome Engineering,College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China [2]College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China [3]School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450002,China [4]Industrial Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China [5]Sichuan Yuliang Testing Co.,Ltd.,Chengdu 611130,China

出　　处：《Journal of Integrative Agriculture》2025年第4期1372-1389,共18页农业科学学报(英文版)

基　　金：supported by the Natural Science Foundation of Henan Province,China(232300421260);the Tackling Key Problems in Science and Technology of Henan Province,China(222102110465,to LZ and 232102111097,to YS);the Open Project Program(SKL-KF202214)。

摘　　要：Maize(Zea mays L.)is a monoecious grass species with separate male and female inflorescences which form the tassel and ear,respectively.The mature ear inflorescences usually bear hundreds of grains,so they directly influence maize grain production and yield.Here,we isolated a recessive maize mutant,tasselseed2016(ts2016),which exhibits pleiotropic inflorescence defects and reduced grain yield.These defects include the loss of determinacy and identity in meristems and floral organs,as well as a lack of the lower floret abortion in maize ear,and a smaller grain size.Using map-based cloning and allelic testing,we identified and confirmed the microRNA gene MIR172e as the target gene controlling these related traits.Furthermore,our evidence uncovered a new potential miR172e/ETHYLENE RESPONSIVE ELEMENT BINDING197(EREB197)regulatory module which controls lower floret abortion in maize ear.Transcriptome analysis revealed that the mutation of MIR172e represses multiple biological processes,particularly the flower development and hormone-related pathways in maize ear.We also found that a mutation in the DNA sequence of MIR172e affects RNA transcription,resulting in elongation blockage at the mutant site.Our results reveal the function and molecular mechanism of MIR172e in maize inflorescences and grain yield,and this study deepens our knowledge of maize inflorescence development.

关 键 词：INFLORESCENCE EAR ts2016 MIR172e grain yield maize(Zea mays L.) 

分 类 号：S513[农业科学—作物学]