Genetic diversity of the self-incompatibility locus in diploid potato  

作　　者：Xinyu Jing Duoduo Qian Xiuhan Jiang Pei Wang Huihui Bao Dianjue Li Yanhui Zhu Guangtao Zhu Chunzhi Zhang 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China [2]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [3]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,China [4]Yunnan Key Laboratory of Potato Biology,The AGISCAAS–YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming 650000,China

出　　处：《Journal of Integrative Agriculture》2025年第4期1448-1460,共13页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(32372695 and 32488302);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAASZDRW202404)。

摘　　要：Re-domestication of diploid potato(Solanum tuberosum)into a seed crop is an innovative breeding method to accelerate genetic improvement.Seed propagation would allow hybrid production and mix superior alleles.However,almost all diploid potatoes in nature are self-incompatible(SI).Gametophytic self-incompatible(GSI)is a widespread SI in Solanaceae and is controlled by the S locus that contains a ribonuclease(S-RNase)and multiple F-box(SLFs);however,the genetic diversity of the S locus in potato is unclear.This study identified 21 S-RNase alleles involved in SI from 194 diploid potato accessions by large-scale transcriptome sequencing.The levels of amino acid similarity among different S-RNase proteins varied from 31.3 to 95.8%.S2 allele is the most widespread in 194 diploid potatoes and is mainly distributed in the S.tuberosum Group Phureja.Based on genomic annotation and expression analysis,we identified 12 potential functional SI male-determinant genes,S-locus F-box(SLFs),encoding F-box proteins in the S2 locus on a genomic region of approximately 13 Mb.Comparative genomics analysis showed that eight SLF genes are relatively conserved among four homozygous S locus.The Ka and Ks analysis suggested that S-RNase and intra-haplotypic SLF genes have co-evolved.These findings help select suitable pollinators,combine more hybrid combinations,and fully use heterosis to accelerate diploid potato breeding.

关 键 词：POTATO SELF-INCOMPATIBILITY S-RNASE SLF 

分 类 号：S532[农业科学—作物学]