Overexpression of the apple m^(6)A demethylase gene MdALKBH1A regulates resistance to heat stress and fixed-carbon starvation  

作　　者：Ru Bao Tianli Guo Zehua Yang Chengyu Feng Junyao Wu Xiaomin Fu Liu Hu Changhai Liu Fengwang Ma 

机构地区：[1]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling 712100,China

出　　处：《Journal of Integrative Agriculture》2025年第4期1489-1502,共14页农业科学学报(英文版)

基　　金：supported by the earmarked fund for the China Agricultural Research System(CARS-27)。

摘　　要：The pivotal role of N^(6)-methyladenosine(m^(6)A)demethylases in regulating plant stress responses has been widely explored;however,the function of apple m^(6)A demethylases under heat stress and fixed-carbon starvation is unclear.In this study,the apple RNA demethylase gene family was identified,and the demethylase gene MdALKBH1A was selected for further analysis.Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis,we demonstrated that MdALKBH1A is the m^(6)A demethylase of apple.Moreover,transgenic‘Micro Tom’tomato plants overexpressing MdALKBH1A were more sensitive to high temperature,probably due to the decreased antioxidant ability,increased membrane lipid peroxidation and reduced plasma membrane stability.However,these tomato plants overexpressing MdALKBH1A were more resistant to fixed-carbon starvation,as evidenced by the improved plasma membrane stability,enhanced photosynthetic rates and elevated autophagic activity.In summary,our results highlight the crucial role played by Md ALKBH1A in the response of apple plants to high-temperature stress and fixedcarbon starvation.

关 键 词：MdALKBH1A heat stress fixed-carbon starvation TOMATO APPLE 

分 类 号：S661.1[农业科学—果树学]