Identification of a TOR signaling pathway gene as a candidate target for reproductive management of Adelphocoris suturalis  

作　　者：Wanying Zheng Panyang Jiao Xiaona Xu Weihua Ma J.Joe Hull Hongxia Hua Lizhen Chen 

机构地区：[1]College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]Pest Management and Biocontrol Research Unit,US Arid Land Agricultural Research Center,USDA Agricultural Research Services,Maricopa,AZ 85138,USA

出　　处：《Journal of Integrative Agriculture》2025年第4期1529-1541,共13页农业科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(32072428)。

摘　　要：Adelphocoris suturalis is a polyphagous pest that is increasingly causing severe economic damage due to more frequent outbreaks.The development of non-target resistance to commercial Bacillus thuringiensis(Bt)cotton has further exacerbated its pest status and amplified the need for more sustainable methods of control.RNA interference(RNAi)-based pest management strategies,such as root soaking and transgenic plants that express dsRNAs,have proven to be reliable,eco-friendly pest control strategies.To identify new RNAi targets for potential A.suturalis population control,we investigated the target of rapamycin(TOR)signaling pathway.A critical role for this pathway in A.suturalis reproductive regulation was suggested by pharmacological analyses.Subsequent RNAi-mediated knockdown of the A.suturalis TOR pathway genes TOR,Ras homolog enriched in the brain(Rheb),and ribosomal S6 kinase(S6K)reduced fertility.Moreover,a spray-induced and nanocarrier-delivered gene silencing(SI-NDGS)system targeting TOR successfully suppressed ovarian development,which demonstrates its effectiveness as a pest control target.These results provide a critical foundation for understanding reproductive regulation in A.suturalis and introduce new candidates for RNAi-based A.suturalis management.

关 键 词：Adelphocoris suturalis target of rapamycin(TOR)signaling reproductive regulation RNAi targets nanocarrier-mediated RNAi 

分 类 号：S433[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]