独脚金内酯对冷藏期‘珍珠油杏’果实品质的调控  

Regulation by strigolactone on the quality of‘Zhenzhuyouxing’apricot fruit during cold storage

作　　者：刘敏[1] 黄丹丹 王元一 丁婴 孟庆喜[1] 朱树华[1] LIU Min;HUANG Dandan;WANG Yuanyi;DING Ying;MENG Qingxi;ZHU Shuhua(College of Chemistry and Materials Science,Shandong Agricultural University,Taian 271018,Shandong,China;State-owned Lianhuashan Forest Farm,Xintai 271200,Shandong,China)

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018 [2]新泰市国有莲花山林场,山东新泰271200

出　　处：《经济林研究》2025年第1期232-241,共10页Non-wood Forest Research

基　　金：山东省乡村振兴科技创新提振行动计划项目(2022TZXD0023);国家自然科学基金项目(32071808)。

摘　　要：【目的】探究不同浓度独脚金内酯(SL)对‘珍珠油杏’果实品质及钾钠稳态的影响,并筛选出维持杏果实品质的最适SL浓度。【方法】分别用0、0.5、1.0、2.0、4.0μmol/L SL以及5.0μmol/L独脚金内酯合成抑制剂(Tis-108)对杏果实进行浸果处理后于0℃贮藏,测定了不同处理对果实硬度、相对电导率、亮度和可溶性固形物质量分数等杏果实品质指标的影响,筛选出适宜杏果实贮藏的SL浓度;进一步测定了杏果实腐胺、亚精胺和精胺质量比及ATP质量比、H^(+)-ATPase、H^(+)-PPase活性和钠、钾离子质量比变化,以探究SL处理对杏果实多胺含量、能量代谢和钾钠稳态的影响。采用单因素方差分析检验各处理间差异显著性。【结果】与对照处理及其它浓度处理相比,2.0μmol/L SL处理通过多途径协同作用显著延缓了采后杏果实的成熟衰老进程。2.0μmol/L SL有效抑制了果实呼吸速率升高、可溶性固形物的积累以及硬度下降,抑制了杏果实质量的减少,同时抑制杏果实相对电导率的上升,使相对电导率维持在一个稳定水平。通过抑制ATP质量比下降及延缓H^(+)-ATPase、H^(+)-PPase活性的降低,2.0μmol/L SL处理能保障细胞膜能量代谢稳定。2.0μmol/L SL能调降低冷藏期间杏果实钠离子含量、提高钾离子含量,维持钾钠动态平衡。同时,2.0μmol/L SL提高了杏果实内腐胺、精胺和亚精胺3种多胺的质量比,高水平的多胺有利于保持果蔬采后品质和延长贮藏期。【结论】2.0μmol/L的SL浸果处理通过整合能量代谢调控、离子稳态维持及膜系统保护机制,显著延缓了‘珍珠油杏’果实在0℃低温贮藏期的品质下降,使杏果实在采后20d内保持良好的贮藏品质。【Objective】In order to explore the effects of different concentrations of strigolactone(SL)on‘Zhenzhuyouxing’apricot fruit quality and potassium sodium homeostasis and to screen out the optimal concentration of SL to maintain apricot fruit quality.【Method】Apricot fruits were soaked in 0,0.5,1.0,2.0,4.0μmol/L SL and 5.0μmol/L strigolactone(Tis-108)and stored at 0℃.The effects of different treatments on fruit firmness,relative conductivity,brightness and soluble solid mass fraction were measured to screen the appropriate SL concentration for apricot fruit storage.Further,the changes in the mass ratio of putrescine,spermidine,spermine and ATP,H^(+)-ATPase and H^(+)-PPase activities,and the mass fraction of potassium and sodium ion contents were determined to investigate the effects of SL treatment on polyamine mass fraction,energy metabolism and potassium-sodium homeostasis in apricot fruits.One-way ANOVA was used to test the significance of differences among treatments.【Result】Compared with the control treatment and other concentration treatments,SL at 2.0μmol/L significantly delayed the postharvest ripening and senescence process of apricot fruits through multiple pathways.The 2.0μmol/L SL effectively inhibited the increase in fruit respiration rate,the accumulation of soluble solids,and the decrease in firmness,prevented the reduction in fruit mass,and suppressed the increase in relative conductivity of apricot fruits,maintaining it at a stable level.By inhibiting the decline in ATP mass ratio and delaying the reduction in H^(+)-ATPase and H^(+)-PPase activities,SL at 2.0μmol/L could ensure the stability of cell membrane energy metabolism.SL at 2.0μmol/L could reduce the sodium ion mass fraction and increase the potassium ion mass fraction of apricot fruits during cold storage,maintaining the dynamic balance of potassium and sodium.Meanwhile,SL at 2.0μmol/L increased the mass ratio of putrescine,spermidine,and spermine in apricot fruits,which are beneficial for maintaining the postharvest

关 键 词：杏果实 独脚金内酯 果实品质 钾钠稳态 采后冷藏 

分 类 号：S662.2[农业科学—果树学]