掺杂ACP的纳米纤维素/壳聚糖纤维复合膜的构建及性能研究  

Preparation and Properties of ACP-doped CNF/ChNFComposite Fiber Membrane

作　　者：李静[1] 邓霁霞 黄晨[1] 冯妍慧芝 LI Jing;DENG Jixia;HUANG Chen;FENG Yanhuizhi(College of Textiles,Donghua University,Shanghai 201620,China;Department of Implantology,Shanghai Tongji Stomatological Hospital and Dental School,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Tongji Research Institute of Stomatology,Shanghai 200072,China)

机构地区：[1]东华大学纺织学院,上海201620 [2]上海市同济口腔医院种植科,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学口腔医学研究所,上海200072

出　　处：《纤维素科学与技术》2025年第1期14-20,I0002,共8页Journal of Cellulose Science and Technology

基　　金：国家自然基金委青年科学基金项目(82301152);上海市浦江人才计划(D类)(22PJD081)。

摘　　要：采用TEMPO氧化法成功制备了羧基化纳米纤维素(CNF)及纳米壳聚糖(ChNF)作为支撑基质,并实现对无定形磷酸钙(ACP)的诱导矿化,从而推动了牙周组织的再生修复。通过定向冷冻干燥与溶液浇筑工艺的优化结合,制备了CNF/ChNF-ACP双层复合膜,其干态和湿态断裂强度分别达到17.5 MPa和3.8 MPa,显著提升了牙周组织修复支架的力学性能。体外细胞和抗菌实验表明,该双层膜可促进MC3T3-E1细胞增殖,对大肠杆菌和金黄色葡萄菌的抑菌率均超96%,该CNF/ChNF-ACP双层复合膜在口腔牙槽骨再生领域展现出显著的应用潜力,为牙周组织工程提供了新的材料选择。Cellulose nanofibers(CNF)and chitosan nanofibers(ChNF)were successfully prepared by TEMPO oxidation method as supporting matrix and achieved induced mineralization of amorphous calcium phosphate(ACP),which promoted the regenerative repair of periodontal tissues.Through the optimized combination of directional freeze-drying and solution casting processes,CNF/ChNF-ACP bilayer composite membranes were prepared with dry and wet fracture strengths of 17.5 MPa and 3.8 MPa,respectively,which significantly enhanced the mechanical properties of the periodontal tissue restoration scaffolds.In vitro cellular and antimicrobial experiments showed that the bilayer membrane could promote the proliferation of MC3T3-E1 cells,and the inhibition rate of E.coli and Staphylococcus aureus was more than 96%,and the CNF/ChNF-ACP bilayer composite membrane showed significant potential for application in the field of oral alveolar bone regeneration,which provided a new material option for periodontal tissue engineering.

关 键 词：纤维素 壳聚糖 纤维膜 TEMPO氧化法 仿生矿化 

分 类 号：TQ174[化学工程—陶瓷工业]