一种简易的^(14)CO_(2)标记植物单叶光合作用方法研究  

Simple and Convenient Method for Preparing and Collecting ^(14)CO_(2) and Feeding to Single Leaf of Plant

作　　者：龚月桦[1,2] 李守桂 刘欣睿 吕金印[2] GONG Yuehua;LI Shougui;LIU Xinrui;LÜJinyin(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin 644000,China;College of Life Science,Northwest A&F University,Yangling 712100,China)

机构地区：[1]宜宾学院农林与食品工程学部,宜宾644000 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《同位素》2025年第2期191-194,共4页Journal of Isotopes

基　　金：国家自然科学青年基金(30600075);宜宾学院教学改革项目(JGY201811);植物生理学课程思政示范建设项目。

摘　　要：^(14)CO_(2)标记是研究植物光合作用及有机物运输分配常用的方法,但现有单叶^(14)CO_(2)标记方法部分比较复杂,部分资料过于简单,实用性不强。为了深入细致研究光合产物运输分配的源流库关系,需要对叶片进行^(14)CO_(2)饲喂。本研究利用连通器的原理,建立一种简便易行的^(14)CO_(2)制备收集和单叶饲喂^(14)CO_(2)的方法,可为植物单叶光合作用研究提供支持。^(14)CO_(2) labeling is a method commonly used in the study of transport and distribution of photoassimilate in plant.In order to study the source-flow-sink relationship of photosynthate transport and distribution,^(14)CO_(2) labeling of single leaf is required.Some of the methods of singleleaf ^(14)CO_(2) labeling reported in the existing literature are complicated and some of the date are too simple,they are not practical.Therefore,through years of practice,we have established a simple and feasible method for preparing and collecting ^(14)CO_(2) and feeding single leaf using the principle of the connector,which provides necessary support for the study of plant photosynthesis.

关 键 词：^(14)CO_(2) 制备 收集 饲喂 

分 类 号：TL99[核科学技术—核技术及应用] Q945.11[生物学—植物学] Q945.18