不同频率单侧刺激小鼠背侧纹状体D1-MSN对小鼠运动速度的影响  

Regulation of unilateral stimulating striatal D1-MSN with different frequencies on movement in mice

作　　者：成芳园 陈冬琨 刘惠金 贾军[1] 王可[1] Cheng Fangyuan;Chen Dongkun;Liu Huijin;Jia Jun;Wang Ke(Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学基础医学院生理学与病理生理学系,北京100069

出　　处：《首都医科大学学报》2025年第2期283-288,共6页Journal of Capital Medical University

基　　金：国家自然科学基金项目(81873364,81774398)。

摘　　要：目的明确单侧激活小鼠背侧纹状体多巴胺Ⅰ型受体中型多棘神经元(dopamine typeⅠreceptor medium-sized multi-spiny neurons,D1-MSN)对小鼠运动速度的影响。方法通过转基因动物结合光遗传学实验,以不同频率特异性激活小鼠背侧纹状体D1-MSN,分析刺激D1-MSN时小鼠的旋转行为及运动速度。结果单侧刺激D1-MSN会增加小鼠的运动速度并诱发小鼠的对侧旋转行为,且诱发的旋转行为随着刺激频率的增加而增加。在刺激D1-MSN未诱发旋转行为的实验中发现5 Hz刺激仍可引起小鼠运动速度增加,但15 Hz,25 Hz刺激并未引起小鼠的运动速度增加。进一步分析小鼠刺激前的运动状态,结果发现刺激前平均运动速度小于5 cm/s时,5 Hz、15 Hz和25 Hz刺激均可增加运动速度,但对刺激前平均运动速度大于5 cm/s时,15 Hz刺激却降低了小鼠运动的速度。结论单侧激活背侧纹状体D1-MSN可调节小鼠的运动速度及诱发小鼠的对侧旋转行为,且与刺激频率和刺激前小鼠的运动状态有关。Objective To clarify the effect of unilateral activation of the dopamine type I receptor medium-sized multi-spiny neurons(D1-MSN)in the dorsal striatum of mice on speed.Methods The transgenic animals were combined with optogenetic experiments to specifically activate the D1-MSN in the dorsal striatum of mice at different frequencies and to analyze the rotational behavior and speed of mice when stimulating the D1-MSN.Results Unilateral activation of D1-MSN induces contralateral rotational behavior in mice and either increases or decreases speed.The mechanisms by which different frequencies affect the speed of mice differently.As the frequency of stimulus increased,the contralateral rotational behavior of the mice increased.Unilateral stimulus of D1-MSN increased speed and induced contralateral rotational behavior,and the rotational behavior increased with increasing stimulus frequency.In the experiment where D1-MSN stimulus did not induce rotational behavior,it was found that 5 Hz stimulus still induced an increase in speed,but 15 Hz and 25 Hz stimulus did not induce an increase in speed.Further analysis of the pre-stimulus locomotor state of the mice showed that 5 Hz,15 Hz and 25 Hz stimulus increased speed when the average speed before stimulus was less than 5 cm/s.However,15 Hz stimulus decreased the speed when the average speed before stimulus was greater than 5 cm/s.Conclusion Unilateral activation of D1-MSN in the dorsal striatum modulates speed and induces contralateral rotational behavior in mice,and is related to the frequency of stimulus and the locomotor state of the mice before stimulus.

关 键 词：基底神经节环路 纹状体 中型多棘神经元 旋转行为 运动速度 小鼠 

分 类 号：R339.4[医药卫生—人体生理学]