小鼠铜绿假单胞菌感染诱导脓毒症模型的建立与评价  

Establishment and evaluation of Pseudomonas aeruginosa-induced sepsis model in mice

作　　者：刘薇[1] 王静怡 郑爔 韩悦[1] 李文雄[1] 张进 Liu Wei;Wang Jingyi;Zheng Xi;Han Yue;Li Wenxiong;Zhang Jin(Department of Intensive Care Unit,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院重症医学科,北京100020

出　　处：《首都医科大学学报》2025年第2期324-332,共9页Journal of Capital Medical University

摘　　要：目的建立可靠的铜绿假单胞菌诱导脓毒症模型,为该菌致病性、耐药机制以及感染炎症机制的探讨提供良好的实验方法。方法采用铜绿假单胞菌标准株ATCC 27853为实验菌株,将不同浓度的菌液涂抹在小鼠竖脊肌表面,涂抹后24 h行超声心动图检查心脏功能。留取心、肺、肾组织标本和血标本,检测血清中肌酐、尿素氮、炎症因子以及肌钙蛋白Ⅰ浓度,观察心、肺、肾组织病理改变。结果10^(7)CFU/mL组、10^(8)CFU/mL、10^(9)CFU/mL及10~(10)CFU/mL组感染后24 h生存率分别为100%,93.3%和73.3%和26.7%。铜绿假单胞菌感染组的白细胞介素-6浓度明显高于非感染组。10^(8)CFU/mL组及10^(9)CFU/mL组肿瘤坏死因子-α浓度明显高于非感染组。10^(8)CFU/mL组及10^(9)CFU/mL组左室射血分数较非感染组明显下降,感染组肌钙蛋白Ⅰ浓度较非感染组明显升高。与非感染组相比,仅10^(9)CFU/mL组肌酐及尿素氮水平有明显统计学差异,其他感染组未见明显差异。组织病理学显示,10^(9)CFU/mL组小鼠心、肺、肾组织均受到炎性细胞明显浸润,实质细胞明显水肿,结构排列紊乱,肺泡间隔增厚,肾间质狭窄等。结论实验选取10^(9)CFU/mL细菌浓度成功建立铜绿假单胞菌诱导脓毒症模型,该方法稳定、可靠,可为探讨脓毒症及铜绿假单胞菌感染疾病提供模型基础。Objective To establish a reliable Pseudomonas aeruginosa(PA)-induced sepsis model,providing an effective experimental method for investigating the pathogenicity,antibiotic resistance mechanism and infection-related inflammatory pathways of PA.Methods PA ATCC 27853 was selected as experimental strain.Different concentrations of bacterial suspension were applied to the surface of erector spinae muscle in mice.Echocardiography was performed 24 h after infection to examine cardiac function.Heart,lung,kidney tissue and blood samples were collected.Serum creatinine(Cr),blood urea nitrogen(BUN),inflammatory factors,and cardiac troponinⅠ(CTNI)were detected.The pathological changes in the heart,lung,and kidney tissues were observed.Results The survival rates of the 10^(7)CFU/mL group,10^(8)CFU/mL,10^(9)CFU/mL,and 1010 CFU/mL groups were 100%,93.3%,73.3%,and 26.7%,respectively.The concentration of interleukin-6(IL-6)in the PA-infected group was significantly higher than that in the non-infected group.The concentration of tumor necrosis factor-α(TNF-α)in 10^(8)CFU/mL group and 10^(9)CFU/mL group were significantly higher than that in the non-infected group.The left ventricular ejection fraction(LVEF)of 10^(8)CFU/mL and 10^(9)CFU/mL groups decreased significantly compared to the non-infected group,and the CTNI level increased significantly in infected group compared to the non-infected group.Compared with the non-infected group,only the 10^(9)CFU/mL group showed significant statistical differences in Cr and BUN levels,while no significant differences were observed in the other PA-infected groups.The results of histopathology showed that the heart,lung,and kidney tissues of mice in the 10^(9)CFU/mL group were significantly infiltrated by inflammatory cells,with obvious edema of tissue cells,disordered structural arrangement,thickening of alveolar septa,and renal interstitial stenosis.Conclusion The experiment successfully established a sepsis model induced by PA with a bacterial concentration of 10^(9)CFU/mL,which is stab

关 键 词：铜绿假单胞菌 脓毒症 模型 感染 小鼠 

分 类 号：R459.7[医药卫生—急诊医学]