江汉鸡J亚群禽白血病病毒分离鉴定及致病性分析  

Identification and pathogenicity analysis of subgroup J Avian leukosis virus from Jianghan chicken

作　　者：许云澎 汪最 窦俊峰 李丽 卢琴 金鑫鑫 凌小淳 汪宏才 翟新国 罗青平 李芸轩 XU Yun-peng;WANG Zui;DOU Jun-feng;LI Li;LU Qin;JIN Xin-xin;LING Xiao-chun;WANG Hong-cai;ZHAI Xin-guo;LUO Qing-ping;LI Yun-xuan(School of Life Sciences and Food Engineering,Hebei University of Engineering,Handan056038,Hebei,China;Key Laboratory of Animal Disease Control and Prevention Formulation of Ministry of Agriculture and Rural Affairs,Wuhan430064,China;Hongshan Laboratory,Wuhan430070,China;Hubei Center for Animal Disease Control and Prevention,Wuhan430070,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]农业农村部畜禽细菌病防治制剂创制重点实验室,武汉430064 [3]洪山实验室,武汉430070 [4]湖北省动物疫病预防控制中心,武汉430070

出　　处：《湖北农业科学》2025年第3期112-117,共6页Hubei Agricultural Sciences

基　　金：国家肉鸡产业技术体系项目(CARS-41);种源性疫病防控技术创新研究(G2022027013L);家禽细菌病新型绿色防治制剂的创制及应用(2022hszd005);禽致病性大肠杆菌、沙门氏菌Ⅰ/Ⅵ型分泌系统的致病机制研究(2021CFA019)。

摘　　要：为了解禽白血病病毒(ALV)在江汉鸡中的遗传进化方向和致病性,对来自湖北省武汉市疑似感染ALV的江汉鸡进行病理剖检、病毒分离鉴定,并对分离毒株进行env基因遗传进化分析以及致病力评估。结果表明,病鸡脾脏肿大,肝脏表面肉眼可见肿瘤;匀浆组织接种DF-1细胞后,经ELISA和IFA检测发现P27抗原均呈阳性,PCR亚型鉴定仅扩增出J亚群特异性条带,成功分离出1株ALV-J毒株,命名为HB18449株;分离株env基因的遗传进化分析显示分离株与山东商品蛋鸡分离株SD13QJ03遗传关系最近,且和多数地方鸡分离株一样与英国原型株HPRS103处于同一大分支。此外,致病性试验结果表明,HB18449感染雏鸡会抑制其生长、免疫器官发育、引起病毒血症、降低血液中CD4~+/CD8~+T淋巴细胞比例以及体液免疫应答水平。In order to understand the genetic evolution direction and pathogenicity of Avian leukosis virus(ALV)isolated from Jianghan chickens,pathological dissection,virus isolation and identification on Jianghan chicken suspected of ALV infection in Wuhan,Hubei were conducted.The isolated viral strains underwent genetic evolution analysis of the env gene and pathogenicity evaluation.The post-mortem results revealed splenomegaly in diseased chickens,with visible tumors on the liver surface.Following inoculation of homogeneous tissue onto DF-1 cells,ELISA and IFA assays showed positive P27 antigen findings.Subsequent PCR subtype identification only amplified specific bands for the J subgroup,indicating the successful isolation of an ALV-J strain named HB18449.Genetic evolution analysis of the isolated strain’s env gene demonstrated the closest genetic relationship to the Shandong commercial egg chicken strain SD13QJ03 and clustered with most local chicken isolates in the same major branch as the UK prototype strain HPRS103.Furthermore,pathogenicity experiments revealed that infection with HB18449 inhibited chick growth,impaired immune organ development,induced viremia,decreased the CD4+/CD8+T lymphocyte ratio in blood,and reduced humoral immune response levels.

关 键 词：禽白血病病毒 江汉鸡 遗传进化 致病性 

分 类 号：S831[农业科学—畜牧学]