金沙鲈鲤全长转录组测序分析与抗菌肽基因的鉴定  

Full-length transcriptome sequencing analysis of Percocypris pingi and identification of antimicrobial peptide gene

作　　者：朱思艺 邓龙君 李天才 张扬 郭勇垚 罗伟 杜宗君[1] ZHU Si-yi;DENG Long-jun;LI Tian-cai;ZHANG Yang;GUO Yong-yao;LUO Wei;DU Zong-jun(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu611130,China;Yalong River Hydropower Development Co.,Ltd.,Chengdu610051,China)

机构地区：[1]四川农业大学动物科技学院,成都611130 [2]雅砻江流域水电开发有限公司,成都610051

出　　处：《湖北农业科学》2025年第3期167-175,共9页Hubei Agricultural Sciences

基　　金：四川省科技支撑计划项目(2021YFYZ0015);雅砻江主要经济鱼类及保护性鱼类种群动态研究项目(YLDC-ZBA-2019104)。

摘　　要：为进一步丰富金沙鲈鲤(Percocypris pingi)基因信息数据库,采用PacBio测序平台对金沙鲈鲤进行全长转录组测序。共获得499 007个全长非嵌合序列(Full-length non-chimeric read,FLNC),通过聚类和去冗余获得70 321个isoforms,isoforms的平均长度和N50分别为1 882 bp、2 243 bp。将isoforms与NR、GO、KEGG、KOG和Swiss-Prot 5个公共数据库进行比对,成功注释了57 521个isoforms,并鉴定到2 310个TF、16 225个LncRNA和35 314条CDS。鉴定到5个抗菌肽基因,包括2个hepcidin基因和3个hemoglobin基因。金沙鲈鲤的hepcidin-1、hepcidin-2与多鳞白甲鱼(Onychostoma macrolepis)的hepcidin亲缘关系较近;金沙鲈鲤的hemoglobin-1α与斑马鱼(Danio rerio)的hemoglobin α亲缘关系较近;金沙鲈鲤的hemoglobin-1β与斑点叉尾鮰(Ictalurus punctatus)的hemoglobin 1β、hemoglobin 2β亲缘关系较近;hemoglobin-2β与多鳞白甲鱼的hemoglobin 2β亲缘关系较近。通过对金沙鲈鲤全长转录组的注释分析,阐明了金沙鲈鲤基因参与的生物过程、所在的代谢途径或信号通路等,可以更有效地探究抗菌肽在鱼类免疫中的作用。In order to further enrich the Percocypris pingi gene information database,the PacBio sequencing platform was used to perform full-length transcriptome sequencing of Percocypris pingi.A total of 499007 full-length non-chimeric reads(FLNC)were obtained,and 70321 isoforms were obtained through clustering and redundancy removal.The average length and N50 of the isoforms were 1882 bp and 2243 bp,respectively.By comparing isoforms with five public databases including NR,GO,KEGG,KOG,and Swiss-Prot,57521 isoforms were successfully annotated and 2310 TFs,16225 LncRNAs,and 35314 CDS were identified.Five antimicrobial peptide genes were identified,including two hepcidin genes and three hemoglobin genes.The hepcidin-1and hepcidin-2 of Percocypris pingi was closely related to hepcidin?of Onychostoma macrolepis;the hemoglobin-1αof Percocypris pingi was closely related to hemoglobinα?of Danio rerio;the hemoglobin-1βof Percocypris pingi was closely related to hemoglobin 1βand hemoglobin 2βof Ictalurus punctatus;the hemoglobin-2βof Percocypris pingi was closely related to hemoglobin 2β?of Onychostoma macrolepis.Through annotation analysis of the full-length transcriptome of Percocypris pingi,the biological processes,metabolic pathways,or signaling pathways involved in the Percocypris pingi gene were elucidated,which could more effectively explore the role of antimicrobial peptides in fish immunity.

关 键 词：金沙鲈鲤(Percocypris pingi) 全长转录组 测序 抗菌肽基因 鉴定 

分 类 号：Q917.4[天文地球—古生物学与地层学]