马铃薯Rieske FeS基因克隆及其在干旱胁迫下的表达分析  

Clone of potato Rieske FeS gene and its expression analysis under drought stress

作　　者：叶和军 叶重泽 俞静 赵瑞英 郭志平[3] YE He-jun;YE Chong-ze;YU Jing;ZHAO Rui-ying;GUO Zhi-ping(Lishui Baiyunshan Ecological Forest Farm,Lishui323000,Zhejiang,China;School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou311300,China;College of Ecology,Lishui University,Lishui323000,Zhejiang,China;Lishui No.2 High School,Lishui323000,Zhejiang,China)

机构地区：[1]丽水市白云山生态林场,浙江丽水323000 [2]浙江农林大学林业与生物技术学院,杭州311300 [3]丽水学院生态学院,浙江丽水323000 [4]丽水市第二高级中学,浙江丽水323000

出　　处：《湖北农业科学》2025年第3期176-181,189,共7页Hubei Agricultural Sciences

基　　金：浙江省科技厅公益项目(LGN19C020001)。

摘　　要：以马铃薯(Solanum tuberosum)为研究对象,通过RT-PCR技术克隆马铃薯Rieske FeS基因的cDNA序列,并对该基因进行生信分析;通过荧光定量PCR检测Rieske FeS基因在模拟干旱胁迫(0、12、24 h)下的表达情况。结果表明,马铃薯Rieske FeS基因cDNA序列全长693 bp,包含1个开放阅读框,可编码230个氨基酸,马铃薯Rieske FeS蛋白分子质量为24.27 ku,理论等电点为8.20;Rieske FeS蛋白含有CytB6-F_Fe-S结构域(59~97 aa)、跨膜区(72~94 aa)、Rieske结构域(113~203 aa);马铃薯Rieske FeS蛋白的二级结构中无规则蜷曲占比最高,其次是β-折叠,占比最低的是α-螺旋。系统进化树分析表明,马铃薯Rieske FeS蛋白与烟草Rieske FeS蛋白亲缘关系最接近。qPCR结果显示,马铃薯Rieske FeS基因在所有被检测组织中均有表达,叶片中Rieske FeS基因的相对表达量最高,其次是茎、根,在块茎中相对表达量最低。经PEG干旱胁迫后,叶片中Rieske FeS基因的相对表达量呈先下降后上升趋势,12 h相对表达量显著低于0 h(对照组)。茎段中Rieske FeS基因的相对表达量在模拟干旱处理12 h时显著上调;块茎中模拟干旱胁迫24 h时Rieske FeS基因的相对表达量显著上调;根中各干旱处理间Rieske FeS基因的相对表达量无显著差异。马铃薯Rieske FeS基因与干旱胁迫密切相关。The potato(Solanum tuberosum)was used as the research object.The cDNA sequence of the Rieske FeS gene of the potato was cloned by RT-PCR technology,and bioinformatics analysis was performed on the gene.The expression of the Rieske FeS gene under simulated drought stress(0,12,24 h)was detected by fluorescence quantitative PCR.The results showed that the full-length cDNA sequence of the potato Rieske FeS gene was 693 bp,containing one open reading frame and encoding 230 amino acids.The molecular weight of the potato Rieske FeS protein was 24.27 ku,with a theoretical isoelectric point of 8.20;the Rieske FeS protein contained the CytB6-F_Fe-S domain(59~97 aa),transmembrane region(72~94 aa),and Rieske domain(113~203 aa);the secondary structure of potato Rieske FeS protein had the highest proportion of irregular curls,followed byβ-sheets,and the lowest proportion wasα-helices.The phylogenetic tree analysis showed that the potato Rieske FeS protein was most closely related to the tobacco Rieske FeS protein.The qPCR results showed that the Rieske FeS gene of the potato was expressed in all tested tissues,with the highest relative expression level of Rieske FeS gene in leaves,followed by stems and roots,and the lowest relative expression level in tubers.After PEG drought stress,the relative expression level of Rieske FeS gene in leaves showed a decreasing trend followed by an increasing trend,and the relative expression level at 12 hours was significantly lower than that at 0 hours(control group).The relative expression level of Rieske FeS gene in stem segments was significantly upregulated after 12 hours of simulated drought treatment;the relative expression level of Rieske FeS gene was significantly upregulated in tubers under simulated drought stress for 24 hours;there was no significant difference in the relative expression levels of Rieske FeS gene among the drought treatments in the roots.The potato Rieske FeS gene was closely related to drought stress.

关 键 词：马铃薯(Solanum tuberosum) Rieske FeS基因 克隆 生信分析 干旱胁迫 基因表达 

分 类 号：S532[农业科学—作物学] Q789[生物学—分子生物学]