HPLC法测定孔雀草中万寿菊素含量  

Determination of Patuletin Content in Tagetes patula L.by HPLC

作　　者：刘剑寒 茅加伟 康靖杰 窦德强[1] 陈晓霞[1] LIU Jianhan;MAO Jiawei;KANG Jingjie;DOU Deqiang;CHEN Xiaoxia(Department of Pharmacology,Liaoning University of Traditional Chinese Medicine,Liaoning Dalian 116000,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116000

出　　处：《广州化工》2025年第6期102-105,共4页GuangZhou Chemical Industry

基　　金：辽宁省教育厅揭榜挂帅项目(LJKFZ20056)。

摘　　要：建立了HPLC测定孔雀草茎叶中万寿菊素的含量的方法。10批样品经石油醚脱色处理后甲醇热回流提取,采用Dikma Technologies Diamonsil(5μm,200 mm×4.6 mm)色谱柱,以乙腈-0.3%磷酸水为流动相,梯度洗脱;流速为1.0 mL·min^(-1);检测波长为358 nm;在室温条件下进行,测定其中万寿菊素的含量。万寿菊素标准品在进样量为0.90625~18.125μg·mL^(-1)的范围时线性关系良好(R=0.9996),精密度,稳定性以及重复性良好,其RSD均小于1.5%,平均加样回收率为96.52%,RSD为1.07%,符合测定要求。该研究建立的方法简单,快速灵敏,结果准确,专属性强,可用于孔雀草茎叶中万寿菊素的含量测定,可为今后孔雀草质量标准建立提供试验方法。A HPLC method for determining the content of patuletin in the stems and leaves of Tagetes patula L.was established.After decolorization with petroleum ether,10 batches of samples were extracted by methanol reflux.Using Dikma Technologies Diamond(5μm,200 mm×4.6 mm)chromatography column,with acetonitrile 0.3%phosphoric acid water as the mobile phase,gradientelution.The flow rate was 1.0 mL/min.The detection wavelength was 358 nm.The content of Tagetes patula L.under room temperature was measured.The patuletin standard had a good linear relationship in the range of 0.90625 to 18.125μg∙mL^(-1)(R=0.9996),with good precision,stability and repeatability,and its RSD was less than 1.5%.The average recovery was 96.52%,with RSD was 1.07%,which met the determination requirements.The method is simple,rapid,sensitive and specific,suitable for the determinationof patuletin content in the stems and leaves of Tagetes patula L.,it can provide experimental method for the establishment of quality standards for Tagetes patula L.in the future.

关 键 词：孔雀草 万寿菊素 HPLC 

分 类 号：R917[医药卫生—药物分析学]