牛LncRNA FABP3 AU基因克隆、生物信息学分析及组织表达研究  

Cloning,Bioinformatics Analysis and Tissue Expression of LncRNA FABP3 AU in Cattle

作　　者：许晓改 樊冰冰 余磊[1] 孟聖博 李明[1] 许会芬 Xu Xiaogai;Fan Bingbing;Yu Lei;Meng Shengbo;Li Ming;Xu Huifen(College of Science and Technology,Henan Agricultural University,Zhengzhou,450046,Henan)

机构地区：[1]河南农业大学动物科技学院,河南郑州450046

出　　处：《中国畜禽种业》2025年第3期45-53,共9页The Chinese Livestock and Poultry Breeding

基　　金：河南省现代农业产业技术体系建设专项(HARS-22-13-G1)。

摘　　要：该实验旨在克隆夏南牛脂肪酸结合蛋白3反义非编码长链RNA(Fatty acid-binding protein 3 antisense upstream,lncRNA FABP3 AU)基因的全长序列,并进行生物信息学分析,以探究lncRNA FABP3 AU在不同月龄的夏南牛心脏、脾脏、肺脏、肾脏、肝脏、回肠、背最长肌、皮下脂肪、瘤胃组织的表达规律。以夏南牛皮下脂肪组织中提取lncRNA FABP3 AU经反转录获得cDNA模板,PCR扩增lncRNA FABP3 AU的全长序列,运用在线软件对lncRNA FABP3 AU进行生物信息学分析;通过实时荧光定量PCR方法分别检测lncRNA FABP3 AU在初生、12月龄和24月龄夏南牛的不同组织中的表达情况。结果表明:lncRNA FABP3 AU全长序列1151 bp,生物信息学分析发现lncRNA FABP3 AU不具有编码能力,主要定位于细胞核中,二级结构中有多个茎环结构。实时荧光定量PCR显示,lncRNA FABP3 AU在新生牛、12月龄、24月龄夏南牛各组织中均有表达,在不同月龄的夏南牛组织中脾脏的表达量显著高于其他组织(P<0.05),24月龄夏南牛肝脏组织中lncRNA FABP3 AU的表达量显著低于新生牛(P<0.05)。可见,实验成功克隆了lncRNA FABP3 AU基因的全长序列,并发现其不具备编码能力,24月龄夏南牛肝脏组织中lncRNA FABP3 AU的表达量显著低于新生牛。The aim of this experiment is to clone the full-length sequence of the antisense long non-coding RNA gene of fatty acid-binding protein 3(LncRNA FABP3 AU)in Xianan cattle,and conduct bioinformatics analysis,so as to explore the expression patterns of lncRNA FABP3 AU in the heart,spleen,lung,kidney,liver,ileum,dorsal muscle,subcutaneous fat and rumen tissues of Xianan cattle at different months of age.LncRNA FABP3 AU was extracted from adipocytes of Xianan cattle by reverse transcription to obtain cDNA template,and the full-length sequence of lncRNA FABP3 AU was amplified by PCR,and bioinformatic analysis of lncRNA FABP3 AU was performed by online software;Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA FABP3 AU in different tissues of calf(0 month old),12-month-old and 24-month-old Xianan cattle,respectively.The full-length sequence of LncRNA FABP3 AU was 1151 bp.Bioinformatics analysis showed that lncRNA FABP3 AU had no coding ability and was mainly located in the nucleus with multiple stem ring structures in the secondary structure.Real-time fluorescence quantitative PCR showed that lncRNA FABP3 AU was expressed in newborn cattle,Xianan cattle at 12 months of age and 24 months of age,and the expression level in spleen of Xianan cattle at different months of age was significantly greater than that in other tissues(P<0.05).The expression level of lncRNA FABP3 AU in liver tissues of 24-month-old Xianan cattle was significantly lower than that of newborn cattle(P<0.05).In this experiment,the full length sequence of lncRNA FABP3 AU gene was successfully cloned,and it was found that the lncRNA FABP3 AU gene did not have the coding ability,and the expression level of lncrNA FABP3 AU in liver tissues of 24-month-old Xianan cattle was significantly lower than that of newborn cattle.

关 键 词：夏南牛 LncRNA FABP3 AU 生物信息学分析 编码能力 组织表达 

分 类 号：S831[农业科学—畜牧学]