福建省某规模化猪场猪繁殖与呼吸综合征的净化  

Purification of Porcine Reproductive and Respiratory Syndrome in Large-scale Swine Farms in Fujian Province

作　　者：金艳冬 Jin Yandong(Sanming Animal Disease Prevention and Control Center,Sanming,365001,Fujan)

机构地区：[1]三明市动物疫病预防控制中心,福建三明365001

出　　处：《中国畜禽种业》2025年第3期104-113,共10页The Chinese Livestock and Poultry Breeding

摘　　要：为了净化福建省某规模化猪场猪繁殖与呼吸综合征疾病,该试验对临床发病的猪血清提取核酸后,通过荧光定量PCR、RT-PCR、ORF5基因测序等实验方法进行病原检测,使用DNAStar软件与14种参考序列进行ORF5比对确定毒株类型。采集发病猪血清及检测常见病原后,通过敏感性实验和建立标准曲线确定发病猪血清病毒量。将采集的血清按1∶50、1∶100和1∶150比例稀释后分3组对21头试验猪肌注,通过测量肌注后10 d内试验猪的体温和第30、60、90天PRRSV抗体变化后确定血清驯化最佳稀释比例。结果显示:本场分离毒株ORF5序列与参考序列比对后确定感染为JXA1样毒株。病猪血清病原检测PRRSV为阳性,PRV、CSFV、PCV2、ASFV、H1和H3亚型SIV、FMDV阴性。将病毒量为10^(5.4)拷贝/μL的猪血清稀释试验后确定最佳驯化稀释比例为1∶100。将后备母猪转移至新的生产基地、加强生物安全防控、闭群管理、检测淘汰等,同时跟踪母猪血清驯化后35、150、350和420 d PRRSV抗原和抗体,血清驯化后420 d母猪在第2胎时母猪和所产仔猪抗原抗体均为阴性。可见,通过敏感性实验和建立标准曲线确定血清病毒量及通过猪群试验确定血清稀释比例的方法对猪繁殖与呼吸综合征的净化是可行的。In order to purify the disease of porcine reproductive and respiratory syndrome in a large-scale pig farm in Fujian Province,nucleic acid was extracted from the serum of clinically ill pigs,and the pathogen was detected by fluorescence quantitative PCR,RT-PCR,ORF5 gene sequencing and other experimental methods,and DNAStar software was used to compare ORF5 with 14 reference sequences to determine the type of strain.After collecting the serum of diseased pigs and detecting common pathogens,the serum virus load of diseased pigs was determined by sensitivity experiments and the establishment of standard curves.The collected serum was diluted in the ratio of 1:50,1:100 and 1:150 and divided into 3 groups for intramuscular njection into 21 test pigs,and the optimal dilution ratio of serum acclimation was determined by measuring the body temperature of the test pigs within 10 days after intramuscular injection and the changes of PRRSV antibodies at 30,60 and 90 days.The results showed that the JXA1-like strain was determined while isolated strain ORF5 sequence was compared with the reference sequence.After the detection of serum pathogens,PRRSV was confirmed to be positive,and PRV,CSFV,PCV,ASFV,H1 and H3 subtypes and FMDV were negative.Porcine serum with a viral load of 10^(5.4)copies/μL was diluted to test and the purified serum dilution ratio was determined to be 1:100.After acclimation,the gilts were transferred to a new production base,biosecurity prevention and control was strengthened,group closure management,detection and elimination,etc.,and PRRSV antigens and antibodies were tracked at 35,150,350 and 420 d,and the antigen and antibody of the sows and piglets born were negative in the sow second parity after domestication.It can be seen that it is feasible to purify porcine reproductive and respiratory syndrome by determining the amount of serum virus by sensitivity test and establishing standard curve and determining the dilution ratio of serum by pig herd test.

关 键 词：猪繁殖与呼吸综合征 基因 驯化血清 闭群 

分 类 号：S858[农业科学—临床兽医学]