用于粒子图像测速的30×荧光探测显微镜研制  

Development of 30×Fluorescence Detection Microscope for Particle Image Velocimetry Measurement

作　　者：娄泰袁葛 郭程祥 杨童[1] 杨磊[1] 谢洪波[1] LOU Taiyuange;GUO Chengxiang;YANG Tong;YANG Lei;XIE Hongbo(Key Laboratory of Optoelectronics Information Technology(Ministry of Education),School of Precision Instruments and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学精密仪器与光电子工程学院,光电信息技术教育部重点实验室,天津300072

出　　处：《光子学报》2025年第3期196-206,共11页Acta Photonica Sinica

摘　　要：为了解决目前产品化的显微镜无法满足显微粒子图像测速系统对于复消色差长工作距和集成化要求的问题,研究了长工作距复消色差物镜的设计原理,布局照明与成像结构,得到集成化的显微镜系统。采用折射设计和无限远校正结构,设计了一款具有30倍放大率、数值孔径为0.35、工作波长位于可见光波段且工作距离为17.3 mm的平场复消色差显微物镜。其整体成像性能接近衍射极限。基于光学设计进行加工与装调后,使用仪器进行实验。粒子成像与测速实验结果表明,系统可对直径为1μm的粒子清晰成像,测量速度偏差与理论模拟值偏差在6%以内,说明显微镜系统设计具有可行性,集成化的仪器表现良好。To address the specific requirements of particle image velocimetry systems in microscopic imaging applications,particularly focusing on the challenges encountered when commercial microscopes fail to meet the demands for long working distance,high resolution,and integrated design,a novel 30×fluorescence detection microscope with a long-working-distance apochromatic objective lens specifically tailored for micro-PIV measurements is developed.The design process employs refractive design principles and an infinity-corrected structure to ensure high-quality imaging across a broad visible light spectrum(400~800 nm).The illumination system is designed to provide uniform and stable light,which is critical for capturing high-quality fluorescence images.The imaging components,including the objective lens,tube lens,and filters,are meticulously arranged to achieve a compact and user-friendly system.The optical design has been optimized to minimize various aberrations,including chromatic and spherical aberrations,ensuring that the overall imaging performance approaches the diffraction limit.Methods for increasing the working distance and techniques for apochromatic correction are proposed.The main way to increase the working distance is to adjust the focal length and interval of the negative light group and the positive light group.The method of apochromatism is mainly replacing glass materials and combining them.After optimization,a numerical aperture of 0.35,a working distance of 17.3 mm,and a microscopic objective with a magnification of 30 times were formed,which exceeded the limitations of conventional microscopic objectives.Additionally,the mechanical design of the microscope emphasizes robustness and ease of use,with the entire system weighing approximately 17.29 kg and measuring 360 mm×300 mm×300 mm.The entire microscope system is designed to be portable and lightweight,enabling remote operation capabilities.It features excellent sealing properties,allowing it to be deployed in complex environments while minimizing

关 键 词：光学设计与制造 几何光学 显微镜 探测 成像系统 粒子图像测速 

分 类 号：TH742[机械工程—光学工程]