大鼠超广角眼底自体荧光成像  

Ultrawide-angle fundus autofluorescence imaging in rats

作　　者：张紫阳 米允帅 季林 肖昀[1,2] 夏蔚 刘玉龙[4] 张运海[1,2] ZHANG Ziyang;MI Yunshuai;JI Lin;XIAO Yun;XIA Wei;LIU Yulong;ZHANG Yunhai(Division of Life Sciences and Medicine,School of Biomedical Engineering(Suzhou),University of Science and Technology of China,Hefei 230026,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215000,China;The First Affiliated Hospital,Soochow University,Suzhou 215006,China;The Second Affiliated Hospital,Soochow University,Suzhou 215004,China)

机构地区：[1]中国科学技术大学生物医学工程学院(苏州)生命科学与医学部,安徽合肥230026 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215000 [3]苏州大学附属第一医院,江苏苏州215006 [4]苏州大学附属第二医院,江苏苏州215004

出　　处：《红外与激光工程》2025年第3期299-309,共11页Infrared and Laser Engineering

基　　金：江苏省社会发展项目(BE2023723);苏州市临床重点病种诊疗技术专项(LCZX202105);核医科技创新计划资助项目(ZHYLZD2021001);中国宝原科研基金项目(270001);苏州市先进生物医学成像重点实验室项目(SZS2022008)。

摘　　要：眼底病严重威胁人类视力健康,研究大鼠眼底超广角自体荧光成像有助于揭示人类眼底病的发病机制与过程。基于激光扫描共聚焦成像技术,设计并搭建了用于大鼠的超广角眼底自体荧光成像系统,使用488 nm波长激光激发大鼠眼底产生自体荧光,利用优化通光范围的滤光片获取自体荧光信号,使用经过设计的接目物镜与扫描透镜实现瞳孔处90°的扫描视场角(相当于眼底132.3°视场角),采用多幅图像叠加的方法提高图像的信噪比。在搭建的超广角眼底成像系统上,对Sprague Dawley大鼠进行自体荧光成像实验,将三幅眼底原始图像进行叠加平均,得到信噪比为8以上的大鼠超广角眼底自体荧光图像,能满足大鼠自体荧光图像要求。Objective Common human fundus diseases,such as Age-Related Macular Degeneration(AMD),Diabetes Retinopathy(DR),Retinitis Pigmentosa(RP),etc.,affect 60 million people worldwide.They are the main blindness cause of human.The autofluorescence of the fundus is closely related to the metabolism of the fundus.The autofluorescence imaging of the fundus can obtain more detailed and special fundus information,judge in advance the position where fundus diseases may occur,and accurately locate the position where fundus diseases have occurred.Due to the fact that eye fundus diseases often develop from surrounding areas,they are often missed in the early stages of the disease due to insufficient imaging field angles.Therefore,it is hoped that the imaging field angle can reach 90°at the pupil for a larger range of examination of the fundus.It is necessary to establish animal models for diagnosis and study human fundus diseases.As the structure and composition of rat eyes and human eyes are basically the same,rats are often used as animal models for human fundus disease research.Rat models can be used to replace human eyes for numerous studies related to human fundus diseases.At present,there is no method that can be directly used for ultrawide-angle fundus autofluorescence imaging in rats.Therefore,the development of methods and techniques for ultrawide-angle fundus autofluorescence imaging in rats is of positive significance for expanding the research methods of fundus diseases and developing related fundus disease inspection instruments.For this purpose,an ultrawide-angle fundus autofluorescence imaging system for rats was studied,which can perform fundus autofluorescence imaging on rats.Using rats to carry out related research on human fundus diseases can reveal the pathogenesis and process of human fundus diseases,and provide reference for the development of ultrawide-angle fundus equipment.Methods The ultrawide-angle fundus autofluorescence imaging system for rats in this article is based on laser scanning confocal imagin

关 键 词：眼底成像 眼底自体荧光 共聚焦成像 超广角眼底成像 

分 类 号：O439[机械工程—光学工程]