MEBT/MEBO下调SPRY2促进糖尿病大鼠创面EMT过程的机制  

The promotion mechanism of MEBT/MEBO down-regulating SPRY2 in the EMT process of diabetic rat's wound

作　　者：童丹蕾 马卓飞[2] 黄金梅 田馨如 姜艳[2] 唐乾利[1,2] TONG Dan-Lei;MA Zhuo-Fei;HUANG Jin-Mei(Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,Guangxi,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530001 [2]右江民族医学院研究生院、广西高校桂西地区高发病防治研究重点实验室

出　　处：《中国老年学杂志》2025年第6期1346-1352,共7页Chinese Journal of Gerontology

基　　金：2017年国家自然科学基金面上项目(No.81774327);广西医学高层次领军人才培养“139”计划项目资助(No.桂卫科教发[2018]22号);广西自然科学基金(2020GXNSFBA297052);广西中医药大学研究生教育创新计划项目(桂中医大研[2022]11号)(YCBXJ2022015)。

摘　　要：目的通过观察皮肤再生医疗技术(MEBT/MEBO)对糖尿病大鼠创面修复过程中速发育生长因子同源蛋白家族(SPRY)2表达及上皮间质化的情况,探讨MEBT/MEBO在糖尿病创面修复过程中的作用机制。方法84只Wistar雄性大鼠随机分为MEBO组、rb-bFGF组、模型组、对照组各21只。对照组为急性创面模型,MEBO组、rb-bFGF组、模型组为糖尿病创面模型,模型成功建立后,对照组、模型组创面采用生理盐水外敷治疗,MEBO组、rb-bFGF组分别采MEBO和贝复新外敷治疗。各组创面于第3、7、14天分别随机取7只大鼠,采集创面组织标本并保存。随后进行苏木素-伊红(HE)染色,观察各组创面上皮生长情况;免疫荧光、Western印迹和实时荧光定量聚合酶链反应(qRT-PCR)检测创面组织SPRY2、E-钙黏蛋白(cadherin)及N-cadherin等分子表达。结果对照组、MEBO组及rb-bFGF组创面愈合率显著高于模型组(P<0.05),且创面组织HE染色显示再上皮化结果更好;在造模第7天,MEBO组及rb-bFGF组中SPRY2、E-cadherin蛋白及mRNA表达量明显低于模型组,N-cadherin明显高于模型组(P<0.05);第14天,与模型组比较,MEBO组及rb-bFGF组中SPRY2、E-cadherin蛋白及mRNA表达量明显升高,N-cadherin表达量明显降低(P<0.05)。结论MEBT/MEBO能够下调SPRY2并调控上皮间质化过程,促进创面再上皮化,加快糖尿病创面愈合速度。Objective To investigate the mechanism of skin regenerative medical technology(MEBT/MEBO)in the process of diabetic wound repair by observing the expression of the growth factor homologous protein family(SPRY)2 and epithelial-mesenchymal transformation(EMT)in the MEBT/MEBO.Methods84 male Wistar rats were randomly divided into MEBO group,rb-bFGF group,model group,and control group,with 21 rats in each group.The control group was the acute wound model,the MEBO group,rb-bFGF group and model group were the diabetic wound model.After the successful establishment of the model,the wounds of the control group and model group with normal saline,MEBO group and rb-bFGF group with MEBO and rb-bFGF,respectively.Seven rats were randomly selected from each group on the 3rd,7th and 14th day,respectively,and wound tissue specimens were collected and preserved.Then hematoxylin-eosin(HE)staining was performed to observe the epithelial growth of each group.The expressions of SPRY2,E-cadherin and N-cadherin in wound tissue were detected by immunofluorescence,Western blotting and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Results The wound healing rate of the control group,MEBO group and rb-bFGF group was significantly higher than that of the model group(P<0.05),and HE staining showed better re-epithelialization results.On the 7th day of modeling,the protein and mRNA expressions of SPRY2 and E-cadherin in MEBO group and rb-bFGF group were significantly lower than those in model group,while N-cadherin were significantly higher than those in model group(P<0.05).On the 7th day of modeling,compared with model group,the protein and mRNA expressions of SPRY2 and E-cadherin in MEBO group and rb-bFGF group were significantly increased,while the expression of N-cadherin was significantly decreased(P<0.05).Conclusions MEBT/MEBO could down regulate SPRY2 and regulate EMT process,promote wound re-epithelization,and speed up wound healing in diabetes.

关 键 词：皮肤再生医疗技术 糖尿病创面 速发育生长因子同源蛋白家族(SPRY)2 上皮间质转化 

分 类 号：R285.5[医药卫生—中药学]