不同质量比处理牙本质基质/α-半水硫酸钙复合材料浸提液对MC3T3-E1细胞增殖及成骨分化的影响  

Effect of different mass ratio of treated dentin matrix/α-calcium sulfate hemihydrate composite extracts on proliferation and osteogenic differentiation of MC3T3-E1 cells

作　　者：施鹏伟 高永强 王超 刘一鸣[1] SHI Pengwei;GAO Yongqiang;WANG Chao;LIU Yiming(Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052;Department of Periodontology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou450052;Department of Stomatology,Zhengzhou 460 Hospital,Zhengzhou 450007)

机构地区：[1]郑州大学第一附属医院口腔颌面外科,郑州450052 [2]郑州大学第一附属医院牙周科,郑州450052 [3]郑州四六○医院口腔科,郑州450007

出　　处：《北京生物医学工程》2025年第2期171-176,共6页Beijing Biomedical Engineering

基　　金：河南省医学科技攻关计划省部共建重点项目(SBGJ202002075)资助。

摘　　要：目的探讨不同质量比处理牙本质基质(treated dentin matrix,TDM)/α-半水硫酸钙(α-calcium sulfate hemihydrate,α-CSH)复合材料浸提液对MC3T3-E1细胞增殖和成骨向分化的影响。方法制备α-CSH、10%TDM/α-CSH、30%TDM/α-CSH、50%TDM/α-CSH复合材料浸提液,采用不同质量比TDM/α-CSH复合材料浸提液培养MC3T3-E1细胞。采用CCK-8检测培养0 d、1 d、3 d、5 d、7 d时各组细胞的增殖活力,同时采用RT-qPCR和Western blot实验检测各组细胞中成骨分化相关基因,碱性磷酸酶(alkaline phosphatase,ALP)、骨桥蛋白(osteopontin,OPN)、Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)的表达。结果与空白对照组和α-CSH组相比,10%TDM/α-CSH组、30%TDM/α-CSH组、50%TDM/α-CSH组中细胞的增殖活力明显升高(P<0.05)。培养5 d和7 d时,30%TDM/α-CSH组和50%TDM/α-CSH组中MC3T3-E1细胞的增殖活力明显高于10%TDM/α-CSH组(P<0.05);与30%TDM/α-CSH组相比,50%TDM/α-CSH组中MC3T3-E1细胞的增殖活力也明显升高(P<0.05)。与空白对照组和α-CSH组相比,10%TDM/α-CSH组、30%TDM/α-CSH组、50%TDM/α-CSH组MC3T3-E1细胞中ALP、OPN和RUNX2的表达水平显著升高(P<0.05);30%TDM/α-CSH组和50%TDM/α-CSH组中ALP、OPN和RUNX2的表达水平明显高于10%TDM/α-CSH组(P<0.05);与30%TDM/α-CSH组相比,50%TDM/α-CSH组中ALP、OPN和RUNX2的表达水平也明显升高(P<0.05)。结论TDM/α-CSH复合材料浸提液,尤其是50%TDM/α-CSH复合材料浸提液能够显著促进MC3T3-C1细胞的增殖及成骨向分化。Objective To investigate the effects of different mass ratio of treated dentin matrix(TDM)/α-calcium sulfate hemihydrate(α-CSH)composite extracts on the proliferation and osteogenic differentiation of MC3T3-E1 cells.Methodsα-CSH,10%TDM/α-CSH,30%TDM/α-CSH and 50%TDM/α-CSH composite extracts were prepared,and MC3T3-E1 cells were cultured with different mass ratio of TDM/α-CSH composite extracts.CCK-8 was used to detect the proliferation activity of each group of cells at 0,1,3,5,7 days.RT-qPCR and Western blot were used to detect the expression of genes related to osteogenic differentiation,alkaline phosphatase(ALP),osteopontin(OPN),Runt-related transcription factor 2(RUNX2)in cells of each group.Results Compared with the blank control group andα-CSH group,the cell proliferation activity of 10%TDM/α-CSH group,30%TDM/α-CSH group and 50%TDM/α-CSH group were significantly increased(P<0.05).The proliferation activity of MC3T3-E1 cells in 30%TDM/α-CSH group and 50%TDM/α-CSH group were significantly higher than those in 10%TDM/α-CSH group for 5 and 7 days(P<0.05).Compared with 30%TDM/α-CSH group,the proliferation activity of MC3T3-E1 cells in 50%TDM/α-CSH group was also significantly increased(P<0.05).Compared with blank control group andα-CSH group,the expression of ALP,OPN and RUNX2 in MC3T3-E1 cells of 10%TDM/α-CSH group,30%TDM/α-CSH group and 50%TDM/α-CSH group were significantly increased(P<0.05).The expression of ALP,OPN and RUNX2 in 30%TDM/α-CSH group and 50%TDM/α-CSH group were significantly higher than those in 10%TDM/α-CSH group(P<0.05).Compared with 30%TDM/α-CSH group,the expression of ALP,OPN and RUNX2 in 50%TDM/α-CSH group were also significantly increased(P<0.05).Conclusions TDM/α-CSH composite extract,especially 50%TDM/α-CSH composite extract,can significantly promote the proliferation and osteogenic differentiation of MC3T3-C1 cells.

关 键 词：处理牙本质基质 α-半水硫酸钙 MC3T3-E1 增殖 分化 

分 类 号：R318.08[医药卫生—生物医学工程] R78[医药卫生—基础医学]