壮医毛桃血藤颗粒的制备工艺与薄层色谱鉴别研究  

Study on Preparation Process and Thin Layer Chromatographic Identification ofMaotao Xueteng Granules in Zhuang Medicine

作　　者：孔文 方刚[2,3] 池锐 KONG Wen;FANG Gang;CHI Rui(Guangxi University of Chinese Medicine,Nanning 530001,China;Guangxi Higher Education Key Laboratory for the Research of Du-related Diseases in Zhuang Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory for Applied Fundamental Research of Zhuang Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学壮医药学院,广西南宁530001 [2]广西高校壮医毒病研究重点实验室/广西中医药大学,广西南宁530200 [3]广西壮医应用基础研究重点实验室/广西中医药大学,广西南宁530001

出　　处：《中国民族民间医药》2025年第6期29-36,共8页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西中医药多学科交叉创新资助(编号:GZKJ2304);广西高校壮医毒病研究重点实验室(桂教科研[2022]10号);广西壮族自治区卫生健康委员会壮医应用基础研究重点培育实验室项目(广西中医药大学)(桂卫科教发[2020]17号);广西医学高层次骨干人才培养“139”计划资助项目(桂卫科教发[2020]15号)。

摘　　要：目的:优选毛桃血藤颗粒的制备工艺,建立相关薄层色谱(TLC)鉴别方法。方法:以补骨脂素含量和得膏率的综合评分为评价指标,采用单因素试验结合Box-Behnken响应面法考察提取工艺;以颗粒制粒、成型情况为评价指标考察成型工艺。采用TLC法对颗粒中的五指毛桃、覆盆子进行定性鉴别。结果:最佳提取工艺为加水量12倍,浸泡50 min,提取时间70 min,提取2次;最优成型工艺为药辅比1∶1.5,辅料为可溶性淀粉∶甘露醇(1∶1),85%乙醇润湿,制粒,70℃烘干。TLC斑点清晰,阴性无干扰。结论:研究的制备工艺和薄层鉴别方法稳定、可行,可为后期开发生产提供实验基础。Objective To optimize the preparation process of Maotao Xueteng granules and to establish the related thin-layer chromatography(TLC)identification method.Methods The extraction process was investigated by single-factor test combined with Box-Behnken response surface method using the combined scores of the content of psoralen and the rate of obtaining paste as evaluation indexes;The pellet granulation and molding situation was used as an evaluation index to examine the molding process.TLC method to identify Ficus hirta Vahland Rubus chingii Huin the granules.Results The optimum preparation process was as follows:add 12 times of water and soak for 50 min,and decoction twice with one hour for each time;The optimal molding process was to add mixed excipients of soluble starch∶mannitol(1∶1)according to the drug-excipient ratio of 1∶1.5,and add 85%ethanol for granulation,drying at 70℃.TLC results are well spotted and negative without interference.Conclusion The preparation process and thin-layer identification method studied are stable and feasible,which can provide an experimental basis for later development and production.

关 键 词：毛桃血藤颗粒 响应面法 制备工艺 薄层鉴别 

分 类 号：R284.1[医药卫生—中药学]