桃红逐瘀合剂的质量标准研究  

Studies on Quality Specification of Taohong Zhuyu Mixture

作　　者：赖宏强 夏丽珍[1] 丰文杰 赖增发 LAI Hongqiang;XIA Lizhen;FENG Wenjie;LAI Zengfa(Sanming City Integrated Traditional and Western Hospital,Sanming 365001,China;Inspection center of Sanming Cify,Sanming 365000,China)

机构地区：[1]福建省三明市中西医结合医院,福建三明365001 [2]福建省三明市检验检测中心,福建三明365001

出　　处：《中国民族民间医药》2025年第6期58-61,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：三明市科技计划项目(2023-S-175)。

摘　　要：目的:建立桃红逐瘀合剂的质量标准。方法:采用薄层色谱法(TLC)定性鉴别桃红逐瘀合剂中当归、川芎、赤芍;采用高效液相色谱法(HPLC)测定该制剂中的芍药苷含量,色谱柱为ODS-BP柱(4.6 mm×250 mm, 5μm),流动相为甲醇∶0.15%磷酸溶液(25∶75),柱温:35℃;流速:1.0 mL·min^(-1);检测波长:230 nm;进样量为10μL。结果:当归、川芎、赤芍的TLC图斑点清晰,分离度较好,阴性样品无干扰。芍药苷在0.160~1.596μg范围内,线性关系良好(r=0.9999);芍药苷的加样回收率为98.20%,RSD为0.73%(n=5)。结论:该方法简便可行,专属性、重复性好,可作为桃红逐瘀合剂的内控标准。Objective To establish quality specification of Taohong Zhuyu mixture.Methods Radix Angelicae Sinensis,Szechuan Lovage Rhizome and Radix Paeoniae Rubra in Taohong Zhuyu Mixture were qualitatively identified by the thin layer chromatography(TLC).The content of paeoniflorin in the Mixture was determined by the high performance liquid chromatography(HPLC).The chromatographic column was ODS-BP column(4.6 mm×250 mm,5μm)with mobile phase of methanol-0.15%phosphate acid(25∶75)flowing at 1 mL·min^(-1) in a gradient elution,the detection wavelength was 230 nm,the column temperature was 35℃,and the injection volume was 10μL.Results TLC images of Radix Angelicae Sinensis,Szechuan Lovage Rhizome and Radix Paeoniae Rubra showed clear spots,good separation and no interference from the negative samples.The linear ranges of paeoniflorin were 0.160-1.596μg(r=0.9999).The average recoveryies were 98.20%for paeoniflorin,and the RSDs were 0.73%(n=5).Conclusion The method is simple and feasible with good specificity and repeatability,which can be used for the inner quality standard of Taohong Zhuyu Mixture.

关 键 词：桃红逐瘀合剂 薄层色谱法 高效液相色谱法 芍药苷 

分 类 号：R284.1[医药卫生—中药学]