LED 光质对铁皮石斛和金钗石斛组培苗生长及品质的影响  

Influence of LED Light Quality on Growth and Quality of Tissue Culture Plantets ofDendrobium officinale and D. nobile

作　　者：姜勇强 吴瑶 方中健 叶广英 惠俊爱[2] 周荣 王再花 JIANG Yongqiang;WU Yao;FANG Zhongjian;YE Guangying;HUI Jun’ai;ZHOU Rong;WANG Zaihua(Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Lab of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou 510640;College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering/Key Laboratory of Tropical&Subtropical Flowers and Landscape Plants of Guangdong Higher Education Institution,Guangzhou 510225;School of Architecture and Planning,Foshan University,Foshan,Guangdong 528000)

机构地区：[1]广东省农业科学院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室,广州510640 [2]仲恺农业工程学院园艺园林学院/广东省普通高校热带亚热带花卉与园林植物重点实验室,广州510225 [3]佛山大学建筑与规划学院,广东佛山528000

出　　处：《中国农学通报》2025年第9期65-72,共8页Chinese Agricultural Science Bulletin

基　　金：广东省重点领域研发计划项目“康养花卉高效育种技术创新与高功能成分新品种选育”(2022B0202080002);2023年省级乡村振兴战略专项“广东省农作物种质资源普查与收集”(2023-NBH-00-001);2024年度乡村振兴战略专项资金“以农业领域为单元的广东省现代农业产业体系共性关键技术研发创新团队建设项目(设施与装备(种植方向)共享关键技术)”(2024CXTD01);墨脱县林业和草原局项目“墨脱县铁皮石斛等开发利用”(GZFCG2024-19219)。

摘　　要：为提高药用石斛的组培效率和质量水平,以铁皮石斛和金钗石斛为实验材料,探究红(R)、蓝(B)、绿(G)3种颜色组合(R6B3G0、R6B3G1、R6B3G3、R3B6G0、R3B6G1、R3B6G3、R3B3G3)以及白光(W)等8种不同LED光质组合对石斛增殖、生根与活性成分含量的影响。结果表明,在R3B6G3的光质下铁皮石斛、金钗石斛增殖效果最好,增殖系数分别为6.67、1.59,比对照(W)明显提高72.8%、101.3%,在该处理下金钗石斛鲜重最大。在R6B3G3组合光质处理下铁皮石斛、金钗石斛生根效果最佳,单株根数分别为10.56、9.42根/株,分别比W处理提高了59.5%、75.4%;此时铁皮石斛鲜重、干重最大,分别为1.254、0.135 g/株,比W明显提高81.7%、1.08倍。干物质含量以R3B6G1处理最高,比W明显提高29.0%,该光质下多糖含量达到最大值24.61%,总黄酮含量以W最佳,为1.46%。在R6B3G0和R3B3G3光质下铁皮石斛总酚含量最佳,均比W提高1.39倍;这2种光质下金钗石斛中的石斛碱含量达到最佳,均为0.5%。可见,在R3B6下增加较强绿光,有利于铁皮石斛的增殖;而在R3B6下增加较强绿光或R6B3下补充较弱绿光,有利于金钗石斛的增殖。此外,R6B3补充较强绿光处理有利于2种石斛的生根。进一步研究发现,在R3B6下适当补充较弱绿光有利于铁皮石斛干物质和多糖的积累;在R6B3下增加较强绿光有利于金钗石斛干物质和石斛碱的积累。To improve the efficiency and quality level of medicinal Dendrobium tissue culture,this study used D.officinale and D.nobile as test materials to investigate the effects of eight different LED light quality combinations,including red,blue,green,and white light(R6B3G0,R6B3G1,R6B3G3,R3B6G0,R3B6G1,R3B6G3,R3B3G3,W),on the proliferation,rooting,and active component contents of the kinds of Dendrobium.The results showed that both D.officinale and D.nobile had the best proliferation effects under the light quality culture of R3B6G3,with proliferation coefficients of 6.67 and 1.59,respectively,which were significantly increased by 72.8%and 101.3%compared to the control(W),with D.nobile showing the highest fresh weight under this treatment.Under the treatment of R6B3G3,the best rooting effects were observed for both D.officinale and D.nobile,with individual root numbers of 10.56 and 9.42 per plant,respectively,which were increased by 59.5%and 75.4%compared to W.At the same time,it showed the highest fresh and dry weight contents of D.officinale under this treatment,with 1.254 and 0.135 g/plant,respectively,significantly increasing by 81.7%and 1.08 times compared to W.The dry matter content was highest under the R3B6G1 treatment,significantly increasing by 29.0%compared to W,and the polysaccharide content reached its maximum value of 24.61%under this treatment,while the total flavonoid content was highest under W,at 1.46%.Under the treatment of R6B3G0 and R3B3G3,the content of total phenolics was highest of D.officinale,both increasing by 1.39 times compared to W,and the dendrobine contents of D.nobile was optimal,both at 0.5%.In short,it was beneficial for the proliferation of D.officinale adding stronger green light under the treatment of R3B6,while it was beneficial for the proliferation of D.nobile adding stronger green light under R3B6 or weaker green light under R6B3.In addition,it was effective for rooting of two Dendrobium varieties adding weaker green light under the treatment of R6B3.Furthermore,it was conducive

关 键 词：LED光质 铁皮石斛 金钗石斛 组织培养 增殖效果 生根效果 干物质含量 活性物质含量 

分 类 号：S567.23[农业科学—中草药栽培]