外源抗坏血酸缓解阿特拉津对甜菜幼苗毒性及降解机理研究  

Study on Exogenous Ascorbic Acid Alleviating Toxicity of Atrazine to Beet Seedlings and Its Degradation Mechanism

作　　者：佟盟露 丁玉玲 姜炳杉 王子辰 庞彩卫 周芹[1,2] TONG Menglu;DING Yuling;JIANG Bingshan;WANG Zichen;PANG Caiwei;ZHOU Qin(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080;Inspection and Testing Center of Beet Quality,Ministry of Agriculture and Rural Affairs,Harbin 150080)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080 [2]农业农村部甜菜品质检验测试中心,哈尔滨150080

出　　处：《中国农学通报》2025年第9期157-164,共8页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设项目“质量安全与营养品质评价”(CARS-17);《农药产品中有效成分测定通用分析方法-液相色谱法》体系建设“啶磺草胺等10个农药品种液相色谱分析方法建立”(14192195)。

摘　　要：为了探讨外源抗坏血酸对阿特拉津胁迫下甜菜幼苗毒性的缓解作用及阿特拉津的降解机理。以甜菜‘KWS1197’种子为试验材料,采用盆栽实验与仪器分析相结合的方法,测定不同浓度抗坏血酸对阿特拉津胁迫下甜菜生理生化指标的影响以及土壤中农药残留量及降解机理。结果表明,阿特拉津胁迫抑制了甜菜幼苗的生长,喷施抗坏血酸后对阿特拉津胁迫下甜菜幼苗的毒性有所缓解,其中喷施1 mmol/L抗坏血酸效果最为显著,地上部分和地下部分的干重分别是只喷施阿特拉津的处理组(AT处理组)的2.54倍和3.43倍;叶绿素含量增加了42.5%;MDA含量和电解质渗透率仅为AT处理组的53.57%和65.01%,SOD、POD和CAT酶活性分别比AT处理组降低60.5%、61.3%和51.1%。且喷施抗坏血酸可以减少土壤中阿特拉津的残留量,浓度越高,降解效果越明显,并发现了4个降解产物,进一步推测了阿特拉津4种可能的降解途径。抗坏血酸可改善阿特拉津对甜菜幼苗生长造成的不利影响,1 mmol/L时效果显著,本研究为阿特拉津的降解提供了可靠的参考。In order to explore the alleviating effect of exogenous ascorbic acid on the toxicity of beet seedlings under atrazine stress and the degradation mechanism of atrazine,using beet'KWS1197'seeds as experimental materials,pot experiments combined with instrumental analysis were conducted to determine the effects of different concentrations of ascorbic acid on physiological and biochemical indexes of beet under atrazine stress,as well as the pesticide residues and degradation mechanisms in the soil.The results showed that atrazine stress inhibited the growth of beet seedlings,after spraying ascorbic acid,the toxicity of beet seedlings under atrazine stress was alleviated.Spraying 1mmol/L ascorbic acid had the most significant effect.The dry weight of the above-ground part and the underground part was 2.54 times and 3.43 times that of atrazine treatment group(AT treatment group).Chlorophyll content was increased by 42.5%.MDA content and electrolyte permeability were 53.57%and 65.01%of those in AT treatment group.SOD,POD and CAT enzyme activities were reduced by 60.5%,61.3%and 51.1%,compared with AT treatment group.Spraying ascorbic acid can reduce the residual amount of atrazine in soil,and the higher the concentration,the more obvious the degradation effect.Four degradation products were found,and four possible degradation pathways of atrazine were further speculated.Ascorbic acid can improve the adverse effects of atrazine on the growth of beet seedlings,and the effect is significant at 1 mmol/L.This study provides a reliable reference for the degradation of atrazine.

关 键 词：阿特拉津 甜菜 抗坏血酸 降解 毒性 生理指标 

分 类 号：S13[农业科学—农业基础科学]