瓶内接种AM真菌对牡丹组培苗生长发育的影响  

Effects of Arbuscular Mycorrhiza Fungi Inoculated in Vitro on Growth and Development of Tree Peony Vitroplantlets

作　　者：曾端香 于宁 杜娟 曾瑶 王莲英[2] ZENG Duanxiang;YU Ning;DU Juan;ZENG Yao;WANG Lianying(National Academy of Forestry and Grassland Administration,Beijing 102600;College of Landscape Architecture,Beijing Forestry University,Beijing 100083)

机构地区：[1]国家林业和草原局管理干部学院,北京102600 [2]北京林业大学园林学院,北京100083

出　　处：《中国农学通报》2025年第7期55-60,共6页Chinese Agricultural Science Bulletin

基　　金：国家林业和草原局“百千万人才工程”省部级人选(2020);国家林业和草原局管理干部学院重点课题“全国林草职业院校科技成果转化路径研究”(202315)。

摘　　要：为探索丛枝菌根真菌(AM真菌)对牡丹组培苗菌根化过程和生长发育的影响,以‘乌龙捧盛’牡丹的休眠芽组培的根诱导苗和生根苗以及杂交系‘Z1-WM’和‘Z1-10-3’的胚培养而得的胚根苗为实验材料,在组织培养试管瓶内接种不同种类的AM真菌孢子。结果表明,AM真菌能显著促进牡丹菌根化组培苗的生长发育,瓶内接种AM真菌比对照(未接种)能更有效地促进牡丹上胚轴萌发率,最高达99.5%,且比对照提前30 d以上打破上胚轴休眠,极显著高于GA3、2~4℃低温和对照处理。接种Gg+Gm对‘Z1-10-3’和‘Z1-WM’组培苗的生长增效最显著;接种Gg对‘乌龙捧盛’组培苗的增效作用最大,移栽6个月时的株高、根长、地上部干重、地下部干重、叶面积增长率分别为64.93%、70.90%、35.00%、54.55%、41.15%。因此,AM真菌对牡丹菌根化组培苗生长发育有极显著的增效作用,且缩短了育种进程。To explore arbuscular mycorrhiza(AM) fungi of mycorrhization process and the impact on its growth and development,with tree peony cultivar‘Wulong Pengsheng'for bud-micropropagated vitroplantlets of rooting seedlings and hybrid system‘Z1-WM'and‘Z1-10-3'embryo culture rooting plantlets as the research objects,different kinds of AMF spores in vitro were inoculated.The results showed that active effects of AMF on the growth of mycorrhizal vitroplantlet were investigated.Inoculation of AMF in vitro could increase the transplanting survival ratio of tree peony effectively.The treatments of inoculation of AMF in vitro heightened sprouting rate epicotyl dormancy of plantlets more than CK,and the highest was 99.50%.The time of breaking epicotyl dormancy was 30 d ahead of inoculation of AMF in vitro than that of the control.There was significant difference among inoculation of AMF in vitro and GA3treatment,2-4℃ low temperature and the control.The most significant difference was in‘Z1-WM'and‘Z1-10-3'inoculated with Gg+Gm.As for‘Wulong Pengsheng',after transplantation of 6 months inoculated with Gg,the index was 64.93%,70.90%,35.00%,54.55%,41.15% respectively.Therefore,AM fungi had a significant effect on the growth and development of mycorrhized vitroplantlets of tree peony and shorten the breeding time.

关 键 词：牡丹 瓶内接种 AM真菌 丛枝菌根 菌根化组培苗 打破上胚轴休眠 生长发育 

分 类 号：S685.11[农业科学—观赏园艺]