干旱-复水对烤烟‘YY021’苗期生理指标及干旱相关基因表达量的影响  

Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of‘YY021’Seedlings

作　　者：李丽华[1] 薛冰洁 王亚乐 李雪君[2] 孙焕[2] 孙计平[2] LI Lihua;XUE Bingjie;WANG Yale;LI Xuejun;SUN Huan;SUN Jiping(Luoyang Tobacco Company of Henan Province,Luoyang,Henan 471000;Tobacco Research Institute,Henan Academy of Agricultural Sciences,Xuchang,Henan 461000)

机构地区：[1]河南省烟草公司洛阳市公司,河南洛阳471000 [2]河南省农业科学院烟草研究所,河南许昌461000

出　　处：《中国农学通报》2025年第8期57-62,共6页Chinese Agricultural Science Bulletin

基　　金：河南省烟草公司洛阳市公司科技项目“洛阳烟区新品种选育及主栽品种改良”(2022410300200053)。

摘　　要：‘YY021’是针对旱作烟区选育的烤烟杂交种,为了鉴定其耐旱性,以‘中烟100’为对照品种,采用漂浮育苗干旱-复水试验,通过调查萎蔫指数和含水量、观察活性氧变化、测定丙二醛和脯氨酸含量以及干旱相关基因表达量。结果表明,随着干旱时间延长,‘中烟100’和‘YY021’叶片含水量下降、萎蔫指数增加,活性氧增加,丙二醛含量增加,osmotin基因表达量先降低再增加;干旱7d后,与‘中烟100’相比较,‘YY021’叶片含水量增加4.31%,萎蔫指数降低3.97%,丙二醛降低9.21%,脯氨酸含量增加84.71%;‘YY021’活性氧更低,osmotin基因表达量更高,ERD基因响应更迅速。研究发现,‘YY021’耐旱性优于‘中烟100’,干旱胁迫下,‘YY021’通过积累渗透调节物质脯氨酸以减轻活性氧对膜伤害,ERD和osmotin基因可能是‘YY021’耐旱候选基因。研究探索了‘YY021’耐旱机理,旨在为‘YY021’品种推广提供数据支持。‘YY021’is a hybrid tobacco variety selected for dryland tobacco growing areas.In order to identify the drought tolerance of‘YY021’,‘Zhongyan 100’was used as the control variety,and the droughtrehydration experiment of floating seedling was arranged to investigate wilting index and water content,observe the changes of active oxygen,and measure the content of malonaldehyde,proline and the expression of drought-related gene by qRT-PCR.The results showed that with the prolongation of drought time,the leaf water content of‘Zhongyan 100’and‘YY021’decreased and the wilting index increased.The content of malonaldehyde increased with the extension of drought time.The expression of osmotin gene decreased first and then increased from 0-7 d of drought.After 7 days of drought,compared with‘Zhongyan 100’,the leaf moisture content o‘f YY021’increased by 4.31%,the wilting index of‘YY021’decreased by 3.97%,and the contents of malondialdehyde decreased by 9.21%,and the contents of proline increased by 84.71%.Compared with‘Zhongyan 100’,‘YY021’had lower levels of reactive oxygen species and malondialdehyde,and the higher osmotin gene expression.The ERD gene responded more rapidly.The drought tolerance of‘YY021’was better than that of‘Zhongyan 100’.Under drought stress,the damage of reactive oxygen species to membrane of‘YY021’was reduced by accumulating osmotic adjustment substance proline.ERD and osmotin genes may be candidate genes for drought tolerance of‘YY021’.

关 键 词：烤烟 ‘YY021’ 耐旱性 干旱-复水 含水量 丙二醛 脯氨酸 基因表达 

分 类 号：S572[农业科学—烟草工业]