STRSeqTyperY68二代测序试剂盒与ITO法联用助力侦破一起命案积案  

The Next Generation Sequencing-based STRSeqTyperY68 Kit and ITO Method Assisted in Solving a Cold Homicide Case

作　　者：吴庆玲 伊鹏 陈智 张驰 权宗雪 肖莉 马晶晶 郑蕾 王瑗 王乐 叶健 WU Qingling;YI Peng;CHEN Zhi;ZHANG Chi;QUAN Zongxue;XIAO Li;MA Jingjing;ZHENG Lei;WANG Yuan;WANG Le;YE Jian(The Criminal Investigation Detachment of Linyi Public Security Bureau,Linyi 276000,Shandong,China;People’s Public Security University of China,Beijing 100038,China;Forensic Medicine College of Shanxi Medical University,Jinzhong 030600,Shanxi,China;Institute of Forensic Science,Ministry of Public Security&MPS’Key Laboratory of Forensic Genetics,Beijing 100038,China)

机构地区：[1]临沂市公安局刑侦支队,山东临沂276000 [2]中国人民公安大学,北京100038 [3]山西医科大学法医学院,山西晋中030600 [4]公安部鉴定中心,法医遗传学公安部重点实验室,北京100038

出　　处：《刑事技术》2025年第2期206-210,共5页Forensic Science and Technology

基　　金：国家重点研发计划(2017YFC0803503);北京市现场物证检验工程技术研究中心开放课题(2023CSEEKFKT01)。

摘　　要：目前,基于毛细管电泳技术的Y-STR检案试剂盒单次最多检测约40个Y-STR基因座,仅报告各等位基因分型的长度多态性信息而无法报告其序列信息。STRSeqTyper Y68男性家系精细化排查试剂盒,一次实验可检测1个性别基因座及67个Y-STR基因座,依托MiSeq FGx二代测序平台同时检测Y-STR的长度及序列多态性,从而提高检验效率,为家系精细化区分提供帮助。ITO法是根据孟德尔遗传分离定律计算两个体亲缘关系指数,评估两个体间可能的五级内亲缘关系的方法。联合应用二代测序技术与ITO法,可以有效缩小家系范围。本文报道一起8年未破的强奸命案,通过联合应用STRSeqTyperY68男性家系精细化排查试剂盒检验及ITO法评估现场检材与比对样本间可能的亲缘关系远近,逐步缩小范围,最终案件告破。Currently,capillary electrophoresis-based Y-STR genotyping kits can detect up to 40 Y-STR loci simultaneously.However,these kits only report length-based genotypes and are unable to provide STR sequence information.The STRSeqTyperY68 kit,designed for forensic male pedigree differentiation using next-generation sequencing technology,excels at genotyping 67 Y-STR loci plus a sex-determinant locus in a single-tube reaction on the MiSeq FGx sequencing platform.It simultaneously provides both length and sequence polymorphism genetic information,simplifies testing procedures,enhances effi ciency,and facilitates precise differentiation of male family lineages.The ITO method is commonly used to calculate the kinship index of two individuals’biological relationship based on Mendel’s law of genetic segregation.Additionally,it evaluates consanguineous relationships within five degrees of kinship between two individuals.The combination of next-generation sequencing technology and the ITO method can effectively narrow down the range of potential families.This paper documents a rape and murder case that remained unsolved for eight years.By cooperatively utilizing the STRSeqTyperY68 kit and the ITO method,the potential connection between crime scene evidence and reference samples was evaluated.Gradually,the investigative leads were narrowed down,leading to the resolution of the case.

关 键 词：法医遗传学 二代测序 序列多态性 Y-STR ITO法 

分 类 号：DF795.2[医药卫生—法医学]