芪榆促愈膏对肛瘘术后创面愈合及NF-κB、VEGF表达的影响  

Effect of Qi-Yu pro-healing ointment on wound healing and expressions of NF-κB and VEGF after anal fistula surgery

作　　者：石星 刘访 魏纪中 曹波 李志 许淑敏 董聿锟 肖浩 SHI Xing;LIU Fang;WEI Jizhong;CAO Bo;LI Zhi;XU Shumin;DONG Yukun;XIAO Hao(Guizhou University of Traditional Chinese Medicine,Guiyang 520002,Guizhou,China;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 520001,Guizhou,China;Xingyi People’s Hospital,Xingyi 562400,Guizhou,China;Puding Hospital of Traditional Chinese Medicine,Puding 562100,Guizhou,China)

机构地区：[1]贵州中医药大学,贵州贵阳520002 [2]贵州中医药大学第一附属医院,贵州贵阳520001 [3]兴义市人民医院,贵州兴义562400 [4]普定县中医医院,贵州普定562100

出　　处：《现代中西医结合杂志》2025年第5期611-616,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：贵州中医药大学学术新苗项目(贵科合学术新苗[2023]-01号);贵州省千层次创新型人才项目;贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2024-008);贵州省基础研究计划(自然科学类)项目(黔科合基础-ZK[2023]一般438)。

摘　　要：目的探究芪榆促愈膏对肛瘘术后创面愈合及创面愈合过程中核因子-κB(NF-κB)、血管内皮生长因子(VEGF)表达的影响。方法纳入2021年2—12月在贵州中医药大学第一附属医院肛肠科住院手术的肛瘘患者60例,随机分为实验组及对照组,每组30例。术后实验组给予芪榆促愈膏纱条外敷创面换药,对照组给予凡士林纱条外敷创面换药。观察比较2组术后第3,7,12天创面疼痛、渗液情况和术后第12天创面愈合情况,治疗后第7天分别采用RT-PCR法、Western blot法及免疫组织化学法检测术后第7天创面组织中NF-κB、VEGF mRNA和蛋白表达情况。结果实验组患者术后第3,7,12天换药时疼痛程度均明显轻于同期对照组(P均<0.05),创面渗液均明显少于同期对照组(P均<0.05);实验组术后第12天创面面积明显小于对照组(P<0.05),创面愈合率明显高于对照组(P<0.05);实验组术后第7天创面组织中NF-κB、VEGF mRNA和蛋白相对表达量及二者阳性表达平均光密度值均明显高于对照组(P均<0.05)。结论芪榆促愈膏纱条外敷可有效减轻肛瘘术后患者创面疼痛,减少创面渗液,促进创面愈合,机制可能与上调创面组织中NF-κB、VEGF表达有关。Objective It is to investigate the effect of Qi-Yu pro-healing ointment on wound healing after anal fistula surgery and expressions of nuclear factor-κB(NF-κB)and vascular endothelial growth factor(VEGF)during wound healing.Methods Sixty patients with anal fistula who were hospitalized and operated in the Department of Anus and Intestines of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from February to December 2021 were included,and were randomly divided into experimental group and control group,with 30 cases in each group.The experiment group was treated with external application of Qiyu Pro-healing Ointment for wound dressing,and the control group was treated with external application of van gauze for wound dressing.The wound pain and exudation on the 3rd,7th and 12th day after surgery and wound healing on the 12th day after surgery in the two groups were observed and compared,the mRNA and protein expressions of NF-κB and VEGF in the wound tissues on the 7th day after surgery were respectively detected by RT-PCR,Western blot and immunohistochemistry after treatment for 7 days.Results The pain degrees of patients in the experiment group when wound dressing on the 3rd,7th and 12th days after surgery were obviously less than those of patients in the control group at the same time period(all P<0.05),and the traumatic exudates were significantly less than those in the control group at the same period(all P<0.05);the area of trauma of the experimental group was significantly smaller(P<0.05),while the healing rate of trauma was significantly higher than those of the control group on the 12th day after surgery(both P<0.05);the mRNA and protein relative expressions of NF-κB and VEGF,and the average optical density values of their positive expressions of the experiment group were significantly higher than those of the control group on the 7th day after surgery(all P<0.05).Conclusion External application of Qiyu Pro-healing Ointment can effectively alleviate the complications su

关 键 词：肛瘘 芪榆促愈膏 核因子-ΚB 血管内皮生长因子 创面愈合 

分 类 号：R657.16[医药卫生—外科学]