黄腻苔结直肠癌患者肠道微环境代谢特征研究  

Study on the Metabolic Characteristics of Intestinal Microenvironment in Colorectal Cancer Patients with Yellow Greasy Tongue Coating

作　　者：刘雪丽 吕琨 蒋寒冰[1] 朱跃坤 徐巍 LIU Xueli;LÜKun;JIANG Hanbing;ZHU Yuekun;XU Wei(The First Clinical Medical College,Harbin Medical University,Harbin 150081 Heilongjiang,China;Oncology Department of Integrated Traditional Chinese and Western Medicine,the First Affiliated Hospital of Harbin Medical University,Harbin 150001 Heilongjiang,China)

机构地区：[1]哈尔滨医科大学第一临床医学院,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第一医院中西医结合肿瘤科,黑龙江哈尔滨150001

出　　处：《中医肿瘤学杂志》2025年第2期30-42,共13页Journal of Oncology in Chinese Medicine

基　　金：中西医协同“旗舰”科室建设项目(编号:国中医药综结合函〔2024〕221号);中医优势专科建设项目(编号:黑中医药综涵〔2022〕36号)。

摘　　要：目的本研究试图分析黄腻苔结直肠癌(colorectal cancer,CRC)患者肠道肿瘤微环境的代谢特征。方法随机选取经舌诊仪判读为黄腻苔的CRC患者12例、白腻苔的CRC患者9例,取上述病例术中的癌组织、癌旁组织行代谢组学和转录组学分析。另外后续收集6例黄腻苔和6例白腻苔CRC患者,通过油红O染色、Western blot、PCR、检测MDA、检测ROS实验验证前述结果。结果经代谢组学和转录组学分析发现腻苔CRC患者有脂代谢紊乱特征,其中黄腻苔区别于白腻苔表现为显著的脂肪酸降解途径下调,酰基肉碱是脂肪酸代谢的关键中间产物,其表达水平的上调是黄腻苔CRC患者脂肪酸降解紊乱的反映。结论黄、白腻苔结直肠癌舌苔变化能反映出肠道微环境的代谢特征。Objective To analyze the metabolic characteristics of tumor microenvironment in colorectal cancer(CRC)patients with yellow greasy tongue coating(YGTC).Methods Twelve CRC patients with YGTC and nine patients with white greasy tongue coating(WGTC),as diagnosed by tongue diagnosis instruments,were randomly selected.Cancerous and adjacent non-cancerous tissues from these patients were analyzed using metabolomics and transcriptomics.Additionally,six CRC patients with YGTC and six CRC patients with WGTC were collected,and the previously sequenced results were validated through experiments of Oil Red O staining,Western blot,PCR,MDA detection,and ROS detection.Results Metabolomics and transcriptomics analyses revealed lipid metabolism disorders in CRC patients with greasy tongue coating.The key distinguishing feature of YGTC different from WGTC was the downregulation of fatty acid degradation pathway.Up-regulation of acylcarnitine,a key intermediate in fatty acid metabolism,was observed in CRC patients with YGTC,reflecting disruption of fatty acid degradation.Conclusion The changes in yellow and white greasy tongue coating of CRC patients can reflect the metabolic characteristics of intestinal microenvironment.

关 键 词：结直肠癌 舌苔 代谢组学 转录组学 

分 类 号：R273[医药卫生—中西医结合] R735.3[医药卫生—中医肿瘤科] R378.7[医药卫生—中医学] R363