芪柏愈疡油对糖尿病大鼠溃疡创面作用的疗效及机制研究  

Effect and mechanism of Qibai Yuyang oil on diabetic rat ulcer wound

作　　者：杨旭 方勇[1] 龚旭初[1] 陈海东[1] 郭檬檬 YANG Xu;FANG Yong;GONG Xu-chu(Nantong Hospital of Traditional Chinese Medicine,Nantong,226000,China)

机构地区：[1]南通市中医院(南京中医药大学南通附属医院),南通226000

出　　处：《中国中西医结合外科杂志》2025年第2期277-280,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：南通市基础科学研究和社会民生科技计划项目(JCZ2022118);江苏省中医药科技计划发展项目重点项目(ZD202231)。

摘　　要：目的:应用糖尿病大鼠溃疡模型观察芪柏愈疡油对糖尿病溃疡创面的治疗效果,并研究相关作用机制。方法:将80只SD雄性大鼠随机分为空白对照组(n=16)和糖尿病组(n=64),将后者制备糖尿病大鼠溃疡模型,成模后随机分为模型组、芪柏愈疡油组、复方紫草油组。实验期间记录各组大鼠的创面大小变化。采用HE染色观察创面组织细胞形态学变化。免疫组化染色检测各组大鼠创面组织中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达。结果:芪柏愈疡油组和复方紫草油组的创面愈合率明显高于模型组(P<0.05),芪柏愈疡油组的创面愈合率高于复方紫草油组(P<0.05)。芪柏愈疡油组和复方紫草油组的创面IL-6、TNF-α表达水平明显低于模型组(P<0.05),药物干预后7 d,芪柏愈疡油组创面组织中IL-6的表达明显低于复方紫草油组(P<0.05),芪柏愈疡油组与复方紫草油组的TNF-α表达无明显差异。结论:芪柏愈疡油能够减轻创面炎症反应,降低创面组织中促炎介质IL-6、TNF-α的过表达,进而促进溃疡创面的愈合。Objective To observe the therapeutic effect of Qibai Yuyang oil on diabetic ulcer wound by using diabetic rat ulcer model,and to study the relevant mechanism.Methods Eighty male SD rats were randomly divided into a blank control group(n=16)and a diabetes group(n=64),The latter was used to prepare ulcer model of diabetes rats,After modeling,the mice were randomly divided into model group,Qibai Yuyang oil group,and compound comfrey oil group.The rats were randomly divided into blank control group,model group,Qibai Yuyang oil group and compound comfrey oil group.The changes of wound size were recorded during the experiment.HE staining was used to observe the morphological changes of ulcer wound tissue.The expression of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by immunohistochemical staining.Results The wound healing rate of Qibai Yuyang oil group and compound comfrey oil group was significantly higher than that of model group(P<0.05).The wound healing rate of Qibai Yuyang oil group was higher than that of compound comfrey oil group(P<0.05).The expressions of IL-6 and TNF-αin wound tissue of Qibai Yuyang oil group and compound comfrey oil group were significantly lower than those of model group(P<0.05).At 7 days after drug intervention,the expression of IL-6 in the wound tissue of Qibai Yuyang oil group was significantly lower than that of compound comfrey oil group(P<0.05),but there were no significant differences in the rest.Conclusion Qibai Yuyang oil can reduce the inflammatory reaction of wound,reduce the overexpression of pro-inflammatory mediators IL-6 and TNF-αin wound tissue,and promote the healing of ulcer wound.

关 键 词：芪柏愈疡油 糖尿病溃疡创面 炎症反应 

分 类 号：R641[医药卫生—外科学] R282[医药卫生—临床医学]